Pseudomonas aeruginosa PAO1, PA3002 (mfd)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3002
Name
mfd
Replicon chromosome
Genomic location 3360875 - 3364321 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251692.1
GI 15598198
Affymetrix PA3002_mfd_at
Entrez 880415
GenBank AAG06390.1
INSDC AAG06390.1
NCBI Locus Tag PA3002
protein_id(GenBank) gb|AAG06390.1|AE004725_3|gnl|PseudoCAP|PA3002
TIGR NTL03PA03002
UniParc UPI00000C58C0
UniProtKB Acc Q9HZK3
UniProtKB ID Q9HZK3_PSEAE
UniRef100 UniRef100_Q9HZK3
UniRef50 UniRef50_Q4KFB0
UniRef90 UniRef90_Q9HZK3

Product

Feature Type CDS
Coding Frame 1
Product Name
transcription-repair coupling protein Mfd
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -18.30
Kyte-Doolittle Hydrophobicity Value -0.179
Molecular Weight (kDa) 128.8
Isoelectric Point (pI) 5.71

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 630 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002580 (531 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3002
Search term: mfd

Human Homologs

References

Molecular mechanism of transcription-repair coupling.
Selby CP, Sancar A
Science 1993 Apr 2;260(5104):53-8
PubMed ID: 8465200
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994