Pseudomonas aeruginosa PAO1, PA4024 (eutB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4024
Name
eutB
Replicon chromosome
Genomic location 4505508 - 4506902 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentPart of the PA4022-eat-eutBC operon responsible for ethanolamine catabolism.

Cross-References

RefSeq NP_252713.1
GI 15599219
Affymetrix PA4024_eutB_at
Entrez 879002
GenBank AAG07411.1
INSDC AAG07411.1
NCBI Locus Tag PA4024
protein_id(GenBank) gb|AAG07411.1|AE004819_4|gnl|PseudoCAP|PA4024
TIGR NTL03PA04024
UniParc UPI00000C5BC9
UniProtKB Acc Q9HX03
UniProtKB ID Q9HX03_PSEAE
UniRef100 UniRef100_W0YV40
UniRef50 UniRef50_L7V2J3
UniRef90 UniRef90_Q4K5E2

Product

Feature Type CDS
Coding Frame 1
Product Name
ethanolamine-ammonia lyase, large subunit, EutB
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Charge (pH 7) -15.28
Kyte-Doolittle Hydrophobicity Value 0.022
Molecular Weight (kDa) 50.2
Isoelectric Point (pI) 4.88

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 137 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001659 (529 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4024
Search term: eutB

Human Homologs

References

Functions required for vitamin B12-dependent ethanolamine utilization in Salmonella typhimurium.
Roof DM, Roth JR
J Bacteriol 1989 Jun;171(6):3316-23
PubMed ID: 2656649
Overexpression, purification, and some properties of the AdoCbl-dependent ethanolamine ammonia-lyase from Salmonella typhimurium.
Faust LP, Babior BM
Arch Biochem Biophys 1992 Apr;294(1):50-4
PubMed ID: 1550360
Sequence of Rhodococcus gene cluster encoding the subunits of ethanolamine ammonia-lyase and an APC-like permease.
De Mot R, Nagy I, Schoofs G, Vanderleyden J
Can J Microbiol 1994 May;40(5):403-7
PubMed ID: 8069783
Ethanolamine Catabolism in Pseudomonas aeruginosa PAO1 Is Regulated by the Enhancer-Binding Protein EatR (PA4021) and the Alternative Sigma Factor RpoN.
Lundgren BR, Sarwar Z, Pinto A, Ganley JG, Nomura CT
J Bacteriol 2016 Sep 1;198(17):2318-29
PubMed ID: 27325678