Pseudomonas aeruginosa PAO1, PA5012 (waaF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5012
Name
waaF
Synonym: rfaF
Replicon chromosome
Genomic location 5630850 - 5631887 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_253699.1
GI 15600205
Affymetrix PA5012_waaF_at
Entrez 881422
GenBank AAG08397.1
INSDC AAG08397.1
NCBI Locus Tag PA5012
protein_id(GenBank) gb|AAG08397.1|AE004913_12|gnl|PseudoCAP|PA5012
TIGR NTL03PA05013
UniParc UPI00000D4265
UniProtKB Acc G3XD35
UniProtKB ID G3XD35_PSEAE
UniRef100 UniRef100_W0Z2D8
UniRef50 UniRef50_H3RIE0
UniRef90 UniRef90_A6VDD1

Product

Feature Type CDS
Coding Frame 1
Product Name
heptosyltransferase II
Product Name Confidence: Class 1
Synonyms Heptose II transferase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 3.21
Kyte-Doolittle Hydrophobicity Value -0.126
Molecular Weight (kDa) 38.4
Isoelectric Point (pI) 8.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 298 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004619 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5012
Search term: waaF

Human Homologs

References

Isolation and characterization of two genes, waaC (rfaC) and waaF (rfaF), involved in Pseudomonas aeruginosa serotype O5 inner-core biosynthesis.
de Kievit TR, Lam JS
J Bacteriol 1997 Jun;179(11):3451-7
PubMed ID: 9171387