Pseudomonas aeruginosa PAO1, PA5316 (rpmB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5316
Name
rpmB
Replicon chromosome
Genomic location 5985883 - 5986119 (- strand)

Cross-References

RefSeq NP_254003.1
GI 15600509
Affymetrix PA5316_rpmB_at
DNASU PaCD00008295
Entrez 880891
GenBank AAG08701.1
INSDC AAG08701.1
NCBI Locus Tag PA5316
protein_id(GenBank) gb|AAG08701.1|AE004944_5|gnl|PseudoCAP|PA5316
TIGR NTL03PA05317
UniParc UPI0000133E45
UniProtKB Acc Q9HTN8
UniProtKB ID RL28_PSEAE
UniRef100 UniRef100_A6VEC3
UniRef50 UniRef50_Q6LVN3
UniRef90 UniRef90_C1DK04

Product

Feature Type CDS
Coding Frame 1
Product Name
50S ribosomal protein L28
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 12.91
Kyte-Doolittle Hydrophobicity Value -0.655
Molecular Weight (kDa) 9.1
Isoelectric Point (pI) 12.23

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPD RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate Pseudomonas aeruginosa 3.28 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
6SPG RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from a clinical isolate Pseudomonas aeruginosa 3.34 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
6SPB RIBOSOME 09/01/19 Pseudomonas aeruginosa 50s ribosome from a clinical isolate with a mutation in uL6 Pseudomonas aeruginosa 2.82 ELECTRON MICROSCOPY 100.0
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
6SPF RIBOSOME 09/01/19 Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate Pseudomonas aeruginosa 2.89 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 471 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004887 (528 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5316
Search term: rpmB

Human Homologs

References

Primary structure of protein L28 from the large subunit of Escherichia coli ribosomes.
Wittmann-Liebold B, Marzinzig E
FEBS Lett 1977 Sep 1;81(1):214-7
PubMed ID: 332524
The roles of proteins L28 and L33 in the assembly and function of Escherichia coli ribosomes in vivo.
Maguire BA, Wild DG
Mol Microbiol 1997 Jan;23(2):237-45
PubMed ID: 9044258