Pseudomonas syringae pv. syringae B728a, Psyr_4524

Gene Feature Overview

Strain	Pseudomonas syringae pv. syringae B728a (Feil et al., 2005) GCF_000012245.1|latest
Locus Tag	Psyr_4524
Name
Replicon	chromosome
Genomic location	5390214 - 5391215 (- strand)

Cross-References

RefSeq	YP_237592.1
GI	66047751
EC Number	2.7.7.6
Entrez	3370064
INSDC	AAY39554.1
NCBI Locus Tag	Psyr_4524
UniParc	UPI0000008500
UniProtKB Acc	Q4ZMR8
UniProtKB ID	RPOA_PSEU2
UniRef100	UniRef100_Q48D61
UniRef50	UniRef50_Q4K557
UniRef90	UniRef90_Q4K557

Product

Feature Type	CDS
Coding Frame	1
Product Name	DNA-directed RNA polymerase subunit alpha
Synonyms
Evidence for Translation
Charge (pH 7)	-14.59
Kyte-Doolittle Hydrophobicity Value	-0.195
Molecular Weight (kDa)	36.7
Isoelectric Point (pI)	4.65

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
7XYB	TRANSCRIPTION	06/01/22	The cryo-EM structure of an AlpA-loaded complex	Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT	3.7	ELECTRON MICROSCOPY	95.8
7XL4	TRANSCRIPTION	04/21/22	Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (closed lobe)	Pseudomonas aeruginosa PAO1	3.86	ELECTRON MICROSCOPY	96.0
7XYA	TRANSCRIPTION	06/01/22	The cryo-EM structure of an AlpA-loading complex	Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT	3.3	ELECTRON MICROSCOPY	95.8
7VF9	TRANSCRIPTION	09/10/21	Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes	Pseudomonas aeruginosa PAO1	4.04	ELECTRON MICROSCOPY	96.0
7F0R	TRANSCRIPTION/DNA	06/06/21	Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT	5.8	ELECTRON MICROSCOPY	96.0
7XL3	TRANSCRIPTION	04/21/22	Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (open lobe)	Pseudomonas aeruginosa PAO1	3.13	ELECTRON MICROSCOPY	96.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 589 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001472 (536 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: Psyr_4524 Search term: DNA-directed RNA polymerase subunit alpha

Human Homologs

References