Pseudomonas syringae pv. syringae B728a, Psyr_4549 (rpsJ)

Gene Feature Overview

Strain	Pseudomonas syringae pv. syringae B728a (Feil et al., 2005) GCF_000012245.1|latest
Locus Tag	Psyr_4549
Name	rpsJ
Replicon	chromosome
Genomic location	5403187 - 5403498 (- strand)

Cross-References

RefSeq	YP_237617.2
GI	161486726
Entrez	3370089
INSDC	AAY39579.1
NCBI Locus Tag	Psyr_4549
UniParc	UPI00000084E6
UniProtKB Acc	Q4ZMP3
UniProtKB ID	RS10_PSEU2
UniRef100	UniRef100_Q48D35
UniRef50	UniRef50_Q48D35
UniRef90	UniRef90_Q48D35

Product

Feature Type	CDS
Coding Frame	1
Product Name	30S ribosomal protein S10
Synonyms
Evidence for Translation
Charge (pH 7)	4.71
Kyte-Doolittle Hydrophobicity Value	-0.491
Molecular Weight (kDa)	11.8
Isoelectric Point (pI)	10.47

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
4V57	RIBOSOME	07/21/07	Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin and neomycin.	Escherichia coli	3.5	X-RAY DIFFRACTION	90.3
6X9Q	RIBOSOME,TRANSCRIPTION/TRANSLATION	06/03/20	Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 27 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site	Escherichia coli; SYNTHETIC CONSTRUCT	4.8	ELECTRON MICROSCOPY	90.3
4V7B	RIBOSOME/TRANSLATION	10/27/13	Visualization of two tRNAs trapped in transit during EF-G-mediated translocation	Escherichia coli	6.8	ELECTRON MICROSCOPY	90.3
6XDQ	RIBOSOME,TRANSCRIPTION/TRANSLATION	06/11/20	Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 30 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site	Escherichia coli; SYNTHETIC CONSTRUCT	3.7	ELECTRON MICROSCOPY	90.3
6X7F	RIBOSOME, TRANSCRIPTION/TRANSLATION	05/29/20	Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B2 (TTC-B2) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site	Escherichia coli; SYNTHETIC CONSTRUCT	3.5	ELECTRON MICROSCOPY	90.3
4WOI	RIBOSOME	10/15/14	4,5-linked aminoglycoside antibiotics regulate the bacterial ribosome by targeting dynamic conformational processes within intersubunit bridge B2	Escherichia coli; Escherichia coli str. K-12 substr. MDS42; Thermus thermophilus; SYNTHETIC CONSTRUCT	3	X-RAY DIFFRACTION	90.3
4V55	RIBOSOME	06/17/07	Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin and ribosome recycling factor (RRF).	Escherichia coli; Thermus thermophilus	4	X-RAY DIFFRACTION	90.3
4V56	RIBOSOME	07/21/07	Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin.	Escherichia coli	3.93	X-RAY DIFFRACTION	90.3
4V4H	RIBOSOME	08/04/06	Crystal structure of the bacterial ribosome from Escherichia coli in complex with the antibiotic kasugamyin at 3.5A resolution.	Escherichia coli	3.46	X-RAY DIFFRACTION	90.3
7UNV	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.7	ELECTRON MICROSCOPY	98.1
4V4Q	RIBOSOME	08/30/05	Crystal structure of the bacterial ribosome from Escherichia coli at 3.5 A resolution.	Escherichia coli	3.46	X-RAY DIFFRACTION	90.3
4V9C	Ribosome/Antibiotic	07/25/12	Allosteric control of the ribosome by small-molecule antibiotics	Escherichia coli; SYNTHETIC CONSTRUCT	3.3	X-RAY DIFFRACTION	90.3
7OE1	RIBOSOME	04/30/21	30S ribosomal subunit from E. coli	Escherichia coli (strain K12); Escherichia coli str. K-12 substr. MG1655	3.05	ELECTRON MICROSCOPY	90.3
7UNR	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	98.1
7O19	TRANSLATION	03/29/21	Cryo-EM structure of an Escherichia coli TnaC-ribosome complex stalled in response to L-tryptophan	Escherichia coli K-12; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	90.3
7P3K	RIBOSOME	07/08/21	Cryo-EM structure of 70S ribosome stalled with TnaC peptide (control)	Escherichia coli (strain K12); Escherichia coli K-12	2.9	ELECTRON MICROSCOPY	90.3
6X7K	RIBOSOME,TRANSCRIPTION/TRANSLATION	05/30/20	Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B3 (TTC-B3) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site	Escherichia coli; SYNTHETIC CONSTRUCT	3.1	ELECTRON MICROSCOPY	90.3
6X6T	TRANSCRIPTION	05/29/20	Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B1 (TTC-B1) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site	Escherichia coli; SYNTHETIC CONSTRUCT	3.2	ELECTRON MICROSCOPY	90.3
4V5B	RIBOSOME	11/22/07	Structure of PDF binding helix in complex with the ribosome.	ESCHERICHIA COLI; SYNTHETIC CONSTRUCT	3.74	X-RAY DIFFRACTION	90.3
7UNU	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.9	ELECTRON MICROSCOPY	98.1
7UNW	RIBOSOME	04/11/22	Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B)	Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	2.6	ELECTRON MICROSCOPY	98.1

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name	Source Accession	Source DB	Version	Target Accession	Target Description	Percent Identity	Alignment Length	E-Value
Nitrofurantoin	DB00698	DrugBank	5.1.4	P0A7R5	30S ribosomal protein S10	90.3	103	1.6e-45

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 664 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001446 (528 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: Psyr_4549 Search term: rpsJ Search term: 30S ribosomal protein S10

Human Homologs

References