Pseudomonas aeruginosa UCBPP-PA14, PA14_39020 (eptA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006)
GCF_000014625.1|latest
Locus Tag
PA14_39020
Name
eptA
Replicon chromosome
Genomic location 3478895 - 3480598 (- strand)
Transposon Mutants 2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 4 transposon mutants in orthologs
CommentOverexpression of three P. aeruginosa eptA homologs in E. coli (PA14_39020 , PA14_58610, PA14_21210) revealed that PA14_39020 was able to modify lipid A with phosphoehthanolamine (PMID:25846400)

Cross-References

RefSeq YP_791268.1
GI 116049923
Entrez 4382769
INSDC ABJ11159.1
NCBI Locus Tag PA14_39020
UniParc UPI000045D239
UniProtKB Acc Q02LB8
UniProtKB ID Q02LB8_PSEAB
UniRef100 UniRef100_W0Z324
UniRef50 UniRef50_S2FPC3
UniRef90 UniRef90_Q9I2D3

Product

Feature Type CDS
Coding Frame 1
Product Name
lipid A phosphoethanolamine transferase, EptA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -0.92
Kyte-Doolittle Hydrophobicity Value 0.035
Molecular Weight (kDa) 62.3
Isoelectric Point (pI) 6.83

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 137 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003461 (883 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_39020
Search term: eptA

Human Homologs

References

Extracellular zinc induces phosphoethanolamine addition to Pseudomonas aeruginosa lipid A via the ColRS two-component system.
Nowicki EM, O'Brien JP, Brodbelt JS, Trent MS
Mol Microbiol 2015 Jul;97(1):166-78
PubMed ID: 25846400
Exogenous and Endogenous Phosphoethanolamine Transferases Differently Affect Colistin Resistance and Fitness in Pseudomonas aeruginosa.
Cervoni M, Lo Sciuto A, Bianchini C, Mancone C, Imperi F
Front Microbiol 2021;12:778968
PubMed ID: 34777328
Regulatory Landscape of the Pseudomonas aeruginosa Phosphoethanolamine Transferase Gene eptA in the Context of Colistin Resistance.
Cervoni M, Sposato D, Lo Sciuto A, Imperi F
Antibiotics (Basel) 2023 Jan 18;12(2)
PubMed ID: 36830112
New insights about the EptA protein and its correlation with the pmrC gene in polymyxin resistance in Pseudomonas aeruginosa.
Freire CMADS, Taunay-Rodrigues A, Gonzatti MB, Fonseca FMP, Freire JEDC
Curr Res Microb Sci 2021 Dec;2:100042
PubMed ID: 34841333