Pseudomonas brassicacearum subsp. brassicacearum NFM421, PSEBR_a5073

Gene Feature Overview

Strain	Pseudomonas brassicacearum subsp. brassicacearum NFM421 GCF_000194805.1|latest
Locus Tag	PSEBR_a5073
Name
Replicon	chromosome
Genomic location	6133851 - 6134852 (- strand)

Cross-References

RefSeq	YP_004356513.1
GI	330812051
Entrez	10474274
INSDC	AEA71509.1
NCBI Locus Tag	PSEBR_a5073
UniParc	UPI00003880C4
UniProtKB Acc	F2KLW6
UniProtKB ID	F2KLW6_PSEBN
UniRef100	UniRef100_Q3K612
UniRef50	UniRef50_Q4K557
UniRef90	UniRef90_Q4K557

Product

Feature Type	CDS
Coding Frame	1
Product Name	DNA-directed RNA polymerase subunit alpha
Synonyms
Evidence for Translation
Charge (pH 7)	-14.59
Kyte-Doolittle Hydrophobicity Value	-0.204
Molecular Weight (kDa)	36.6
Isoelectric Point (pI)	4.65

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
7XYA	TRANSCRIPTION	06/01/22	The cryo-EM structure of an AlpA-loading complex	Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT	3.3	ELECTRON MICROSCOPY	96.1
7VF9	TRANSCRIPTION	09/10/21	Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes	Pseudomonas aeruginosa PAO1	4.04	ELECTRON MICROSCOPY	96.4
7XYB	TRANSCRIPTION	06/01/22	The cryo-EM structure of an AlpA-loaded complex	Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT	3.7	ELECTRON MICROSCOPY	96.1
7XL3	TRANSCRIPTION	04/21/22	Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (open lobe)	Pseudomonas aeruginosa PAO1	3.13	ELECTRON MICROSCOPY	96.4
7XL4	TRANSCRIPTION	04/21/22	Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (closed lobe)	Pseudomonas aeruginosa PAO1	3.86	ELECTRON MICROSCOPY	96.4
7F0R	TRANSCRIPTION/DNA	06/06/21	Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT	5.8	ELECTRON MICROSCOPY	96.4

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 589 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001472 (536 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PSEBR_a5073 Search term: DNA-directed RNA polymerase subunit alpha

Human Homologs

References