Pseudomonas denitrificans ATCC 13867, H681_02740 (rpoB)

Gene Feature Overview

Strain	Pseudomonas denitrificans ATCC 13867 GCF_000349845.1|latest
Locus Tag	H681_02740
Name	rpoB
Replicon	chromosome
Genomic location	606316 - 610389 (+ strand)

Cross-References

RefSeq	YP_007655970.1
GI	472323724
EC Number	2.7.7.6
Entrez	14994688
INSDC	AGI22430.1
NCBI Locus Tag	H681_02740
UniParc	UPI0002C4F5FC
UniProtKB Acc	M4XAS7
UniProtKB ID	M4XAS7_PSEDE
UniRef100	UniRef100_M4XAS7
UniRef50	UniRef50_P06173
UniRef90	UniRef90_Q51561

Product

Feature Type	CDS
Coding Frame	1
Product Name	DNA-directed RNA polymerase subunit beta
Synonyms
Evidence for Translation
Charge (pH 7)	-23.35
Kyte-Doolittle Hydrophobicity Value	-0.290
Molecular Weight (kDa)	150828.0
Isoelectric Point (pI)	5.34

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
7XL4	TRANSCRIPTION	04/21/22	Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (closed lobe)	Pseudomonas aeruginosa PAO1	3.86	ELECTRON MICROSCOPY	94.5
7XYB	TRANSCRIPTION	06/01/22	The cryo-EM structure of an AlpA-loaded complex	Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT	3.7	ELECTRON MICROSCOPY	94.5
7VF9	TRANSCRIPTION	09/10/21	Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes	Pseudomonas aeruginosa PAO1	4.04	ELECTRON MICROSCOPY	94.5
7F0R	TRANSCRIPTION/DNA	06/06/21	Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT	5.8	ELECTRON MICROSCOPY	94.5
7XYA	TRANSCRIPTION	06/01/22	The cryo-EM structure of an AlpA-loading complex	Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT	3.3	ELECTRON MICROSCOPY	94.5
7XL3	TRANSCRIPTION	04/21/22	Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (open lobe)	Pseudomonas aeruginosa PAO1	3.13	ELECTRON MICROSCOPY	94.5

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 667 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001441 (533 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: H681_02740 Search term: rpoB Search term: DNA-directed RNA polymerase subunit beta

Human Homologs

References