Pseudomonas aeruginosa PAO1, PA0949 (wrbA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0949
Name
wrbA
Replicon chromosome
Genomic location 1036579 - 1037175 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249640.1
GI 15596146
Affymetrix PA0949_wrbA_at
DNASU PaCD00006436
Entrez 882021
GenBank AAG04338.1
INSDC AAG04338.1
NCBI Locus Tag PA0949
protein_id(GenBank) gb|AAG04338.1|AE004529_4|gnl|PseudoCAP|PA0949
TIGR NTL03PA00950
UniParc UPI00000C51D5
UniProtKB Acc Q9I509
UniProtKB ID NQOR_PSEAE
UniRef100 UniRef100_Q9I509
UniRef50 UniRef50_Q9I509
UniRef90 UniRef90_Q9I509

Product

Feature Type CDS
Coding Frame 1
Product Name
Trp repressor binding protein WrbA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -0.66
Kyte-Doolittle Hydrophobicity Value 0.031
Molecular Weight (kDa) 20.7
Isoelectric Point (pI) 6.79

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Periplasmic Class 1
Additional evidence for subcellular localization
Detetected in a periplasmic protein preparation by spheroplasting by osmotic shock.(PMID:19333994)

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1ZWL PROTEIN BINDING 06/03/05 Structure of WrbA from Pseudomonas aeruginosa in complex with FMN Pseudomonas aeruginosa 2.8 X-RAY DIFFRACTION 100.0
1ZWK PROTEIN BINDING 06/03/05 Structure of WrbA from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.6 X-RAY DIFFRACTION 100.0
2A5L TRANSCRIPTION 06/30/05 The crystal structure of the Trp repressor binding protein WrbA from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.7 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 258 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000915 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0949
Search term: wrbA

Human Homologs

References

A stationary-phase protein of Escherichia coli that affects the mode of association between the trp repressor protein and operator-bearing DNA.
Yang W, Ni L, Somerville RL
Proc Natl Acad Sci U S A 1993 Jun 15;90(12):5796-800
PubMed ID: 8516330
Pseudomonas aeruginosa MdaB and WrbA are water-soluble two-electron quinone oxidoreductases with the potential to defend against oxidative stress.
Green LK, La Flamme AC, Ackerley DF
J Microbiol 2014 Sep;52(9):771-7
PubMed ID: 25085734