Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA3364
|
Name |
amiC
|
Replicon | chromosome |
Genomic location | 3774578 - 3775735 (- strand) |
Transposon Mutants | 1 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_252054.1 |
GI | 15598560 |
Affymetrix | PA3364_amiC_at |
Entrez | 877798 |
GenBank | AAG06752.1 |
INSDC | AAG06752.1 |
NCBI Locus Tag | PA3364 |
protein_id(GenBank) | gb|AAG06752.1|AE004758_9|gnl|PseudoCAP|PA3364 |
TIGR | NTL03PA03364 |
UniParc | UPI0000165ECF |
UniProtKB Acc | P27017 |
UniProtKB ID | AMIC_PSEAE |
UniRef100 | UniRef100_P27017 |
UniRef50 | UniRef50_P27017 |
UniRef90 | UniRef90_P27017 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
aliphatic amidase expression-regulating protein
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -6.74 |
Kyte-Doolittle Hydrophobicity Value | -0.313 |
Molecular Weight (kDa) | 42.8 |
Isoelectric Point (pI) | 5.82 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
1PEA | BINDING PROTEIN | 11/16/95 | AMIDE RECEPTOR/NEGATIVE REGULATOR OF THE AMIDASE OPERON OF PSEUDOMONAS AERUGINOSA (AMIC) COMPLEXED WITH ACETAMIDE | Pseudomonas aeruginosa | 2.1 | X-RAY DIFFRACTION | 98.7 |
1QNL | TRANSFERASE | 10/19/99 | AMIDE RECEPTOR/NEGATIVE REGULATOR OF THE AMIDASE OPERON OF PSEUDOMONAS AERUGINOSA (AMIC) COMPLEXED WITH BUTYRAMIDE | PSEUDOMONAS AERUGINOSA | 2.7 | X-RAY DIFFRACTION | 98.4 |
1QO0 | BINDING PROTEIN | 10/26/99 | Amide receptor of the amidase operon of Pseudomonas aeruginosa (AmiC) complexed with the negative regulator AmiR. | PSEUDOMONAS AERUGINOSA | 2.25 | X-RAY DIFFRACTION | 98.7 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
Acetamide | DB02736 | DrugBank | 5.1.4 | P27017 | Aliphatic amidase expression-regulating protein | 100.0 | 385 | 7.1e-227 |
Butyramide | DB02121 | DrugBank | 5.1.4 | P27017 | Aliphatic amidase expression-regulating protein | 100.0 | 385 | 7.1e-227 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 242 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG002264 (348 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA3364
Search term: amiC
Search term: aliphatic amidase expression-regulating protein
|
Antitermination of amidase expression in Pseudomonas aeruginosa is controlled by a novel cytoplasmic amide-binding protein.
Wilson SA, Wachira SJ, Drew RE, Jones D, Pearl LH
EMBO J 1993 Sep;12(9):3637-42
PubMed ID: 8253087
|
Cloning and DNA sequence of amiC, a new gene regulating expression of the Pseudomonas aeruginosa aliphatic amidase, and purification of the amiC product.
Wilson S, Drew R
J Bacteriol 1991 Aug;173(16):4914-21
PubMed ID: 1907262
|
Crystal structure of AmiC: the controller of transcription antitermination in the amidase operon of Pseudomonas aeruginosa.
Pearl L, O'Hara B, Drew R, Wilson S
EMBO J 1994 Dec 15;13(24):5810-7
PubMed ID: 7813419
|