Pseudomonas aeruginosa PAO1, PA4329 (pykA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA4329
Name
pykA
Synonym: pyk-II
Replicon chromosome
Genomic location 4856960 - 4858411 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253019.1
GI 15599525
Affymetrix PA4329_pykA_at
Entrez 881516
GenBank AAG07717.1
INSDC AAG07717.1
NCBI Locus Tag PA4329
protein_id(GenBank) gb|AAG07717.1|AE004849_4|gnl|PseudoCAP|PA4329
TIGR NTL03PA04330
UniParc UPI00000C5CC1
UniProtKB Acc Q9HW72
UniProtKB ID Q9HW72_PSEAE
UniRef100 UniRef100_W0Z092
UniRef50 UniRef50_P21599
UniRef90 UniRef90_Q02HB2

Product

Feature Type CDS
Coding Frame 1
Product Name
pyruvate kinase II
Product Name Confidence: Class 2
Synonyms Phosphoenolpyruvate kinase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -2.24
Kyte-Doolittle Hydrophobicity Value -0.092
Molecular Weight (kDa) 52.3
Isoelectric Point (pI) 6.66

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6QXL TRANSFERASE 03/07/19 Crystal Structure of Pyruvate Kinase II (PykA) from Pseudomonas aeruginosa in complex with sodium malonate, magnesium and glucose-6-phosphate PSEUDOMONAS AERUGINOSA 2.43 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 614 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003926 (554 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4329
Search term: pykA

Human Homologs

Ensembl 97, assembly GRCh38.p12
pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:9021]
E-value: 2.7e-67
Percent Identity: 36.7
Ensembl 97, assembly GRCh38.p12
pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:9021]
E-value: 2.7e-67
Percent Identity: 36.7
Ensembl 97, assembly GRCh38.p12
pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:9021]
E-value: 2.7e-67
Percent Identity: 36.7
Ensembl 97, assembly GRCh38.p12
pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:9021]
E-value: 2.7e-67
Percent Identity: 36.7

References

Divergent binding sites in pyruvate kinases I and II from Escherichia coli.
Valentini G, Stoppini M, Iadarola P, Malcovati M, Ferri G, Speranza ML
Biol. Chem. Hoppe-Seyler 1993 Jan;374(1):69-74
PubMed ID: 8439398
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994