Pseudomonas aeruginosa PAO1, PA5201

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5201
Name
Synonym: tex
Synonym: yhgF
Replicon chromosome
Genomic location 5853585 - 5855924 (+ strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentTex binds dsRNA, ssRNA, dsDNA, and ssDNA, with a strong preference for ssRNA

Cross-References

RefSeq NP_253888.1
GI 15600394
Affymetrix PA5201_at
Entrez 878507
GenBank AAG08586.1
INSDC AAG08586.1
NCBI Locus Tag PA5201
protein_id(GenBank) gb|AAG08586.1|AE004933_2|gnl|PseudoCAP|PA5201
TIGR NTL03PA05202
UniParc UPI00000C5F4F
UniProtKB Acc Q9HTY8
UniProtKB ID Q9HTY8_PSEAE
UniRef100 UniRef100_Q9HTY8
UniRef50 UniRef50_Q45388
UniRef90 UniRef90_Q9HTY8

Product

Feature Type CDS
Coding Frame 1
Product Name
transcriptional accessory protein Tex
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by iTRAQ (PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -5.13
Kyte-Doolittle Hydrophobicity Value -0.285
Molecular Weight (kDa) 85.0
Isoelectric Point (pI) 6.29

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3BZK RNA BINDING PROTEIN 01/18/08 Crystal Structure of the Tex protein from Pseudomonas aeruginosa, crystal form 2 Pseudomonas aeruginosa 2.3 X-RAY DIFFRACTION 100.0
3BZC RNA BINDING PROTEIN 01/17/08 Crystal Structure of the Tex protein from Pseudomonas aeruginosa, crystal form I Pseudomonas aeruginosa 2.27 X-RAY DIFFRACTION 100.0
2OCE STRUCTURAL GENOMICS, UNKNOWN FUNCTION 12/20/06 Crystal structure of Tex family protein PA5201 from Pseudomonas aeruginosa Pseudomonas aeruginosa PAO1 3.1 X-RAY DIFFRACTION 99.9

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 593 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004785 (532 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5201

Human Homologs

Ensembl 110, assembly GRCh38.p14
S1 RNA binding domain 1 [Source:HGNC Symbol;Acc:HGNC:25521]
E-value: 1.5e-98
Percent Identity: 33.6

References

A new gene locus of Bordetella pertussis defines a novel family of prokaryotic transcriptional accessory proteins.
Fuchs TM, Deppisch H, Scarlato V, Gross R
J Bacteriol 1996 Aug;178(15):4445-52
PubMed ID: 8755871
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Crystal structure and RNA binding of the Tex protein from Pseudomonas aeruginosa.
Johnson SJ, Close D, Robinson H, Vallet-Gely I, Dove SL, Hill CP
J Mol Biol 2008 Apr 11;377(5):1460-73
PubMed ID: 18321528
Tex, a putative transcriptional accessory factor, is involved in pathogen fitness in Streptococcus pneumoniae.
He X, Thornton J, Carmicle-Davis S, McDaniel LS
Microb Pathog 2006 Dec;41(6):199-206
PubMed ID: 16997528