Pseudomonas aeruginosa PAO1, PA5260 (hemC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA5260
Name
hemC
Synonym: popE
Replicon chromosome
Genomic location 5921494 - 5922435 (- strand)

Cross-References

RefSeq NP_253947.1
GI 15600453
Affymetrix PA5260_hemC_at
DNASU PaCD00008268
Entrez 877673
GenBank AAG08645.1
INSDC AAG08645.1
NCBI Locus Tag PA5260
protein_id(GenBank) gb|AAG08645.1|AE004938_7|gnl|PseudoCAP|PA5260
TIGR NTL03PA05261
UniParc UPI000012C488
UniProtKB Acc Q60169
UniProtKB ID HEM3_PSEAE
UniRef100 UniRef100_B7V5F0
UniRef50 UniRef50_Q4K3X3
UniRef90 UniRef90_A6VE37

Product

Feature Type CDS
Coding Frame 1
Product Name
porphobilinogen deaminase
Product Name Confidence: Class 2
Synonyms hydroxymethylbilane synthase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.67
Kyte-Doolittle Hydrophobicity Value -0.085
Molecular Weight (kDa) 33.6
Isoelectric Point (pI) 5.26

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 586 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004841 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5260
Search term: hemC

Human Homologs

Ensembl 97, assembly GRCh38.p12
hydroxymethylbilane synthase [Source:HGNC Symbol;Acc:HGNC:4982]
E-value: 7.8e-60
Percent Identity: 41.9
Ensembl 97, assembly GRCh38.p12
hydroxymethylbilane synthase [Source:HGNC Symbol;Acc:HGNC:4982]
E-value: 7.8e-60
Percent Identity: 41.9
Ensembl 97, assembly GRCh38.p12
hydroxymethylbilane synthase [Source:HGNC Symbol;Acc:HGNC:4982]
E-value: 7.8e-60
Percent Identity: 41.9
Ensembl 97, assembly GRCh38.p12
hydroxymethylbilane synthase [Source:HGNC Symbol;Acc:HGNC:4982]
E-value: 7.8e-60
Percent Identity: 41.9
Ensembl 97, assembly GRCh38.p12
hydroxymethylbilane synthase [Source:HGNC Symbol;Acc:HGNC:4982]
E-value: 7.8e-60
Percent Identity: 41.9
Ensembl 97, assembly GRCh38.p12
hydroxymethylbilane synthase [Source:HGNC Symbol;Acc:HGNC:4982]
E-value: 7.8e-60
Percent Identity: 41.9
Ensembl 97, assembly GRCh38.p12
hydroxymethylbilane synthase [Source:HGNC Symbol;Acc:HGNC:4982]
E-value: 7.8e-60
Percent Identity: 41.9
Ensembl 97, assembly GRCh38.p12
hydroxymethylbilane synthase [Source:HGNC Symbol;Acc:HGNC:4982]
E-value: 7.8e-60
Percent Identity: 41.9

References

Evidence for participation of aspartate-84 as a catalytic group at the active site of porphobilinogen deaminase obtained by site-directed mutagenesis of the hemC gene from Escherichia coli.
Woodcock SC, Jordan PM
Biochemistry 1994 Mar;33(9):2688-95
PubMed ID: 8117733
The Pseudomonas aeruginosa homologs of hemC and hemD are linked to the gene encoding the regulator of mucoidy AlgR.
Mohr CD, Sonsteby SK, Deretic V
Mol. Gen. Genet. 1994 Jan;242(2):177-84
PubMed ID: 8159168