Pseudomonas aeruginosa PAO1, PA4996 (rfaE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4996
Name
rfaE
Replicon chromosome
Genomic location 5612268 - 5613692 (- strand)

Cross-References

RefSeq NP_253683.1
GI 15600189
Affymetrix PA4996_rfaE_at
Entrez 881680
GenBank AAG08381.1
INSDC AAG08381.1
NCBI Locus Tag PA4996
protein_id(GenBank) gb|AAG08381.1|AE004912_7|gnl|PseudoCAP|PA4996
TIGR NTL03PA04997
UniParc UPI00000C5EB9
UniProtKB Acc Q9HUG9
UniProtKB ID HLDE_PSEAE
UniRef100 UniRef100_Q9HUG9
UniRef50 UniRef50_Q7AAQ7
UniRef90 UniRef90_Q9HUG9

Product

Feature Type CDS
Coding Frame 1
Product Name
LPS biosynthesis protein RfaE
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -4.63
Kyte-Doolittle Hydrophobicity Value 0.082
Molecular Weight (kDa) 50.3
Isoelectric Point (pI) 6.20

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 463 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004606 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4996
Search term: rfaE

Human Homologs

References

The rfaE gene from Escherichia coli encodes a bifunctional protein involved in biosynthesis of the lipopolysaccharide core precursor ADP-L-glycero-D-manno-heptose.
Valvano MA, Marolda CL, Bittner M, Glaskin-Clay M, Simon TL, Klena JD
J Bacteriol 2000 Jan;182(2):488-97
PubMed ID: 10629197
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994