Pseudomonas aeruginosa PAO1, PA0157 (triB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0157
Name
triB
Synonym: triB
Replicon chromosome
Genomic location 178455 - 179525 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1

Cross-References

RefSeq NP_248847.1
GI 15595355
Affymetrix PA0157_at
DNASU PaCD00006068
Entrez 882134
GenBank AAG03547.1
INSDC AAG03547.1
NCBI Locus Tag PA0157
protein_id(GenBank) gb|AAG03547.1|AE004453_10|gnl|PseudoCAP|PA0157
TIGR NTL03PA00158
UniParc UPI00000C4F6C
UniProtKB Acc Q9I6X5
UniProtKB ID Q9I6X5_PSEAE
UniRef100 UniRef100_A0A077JP41
UniRef50 UniRef50_G8PZD9
UniRef90 UniRef90_W0YL24

Product

Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) triclosan efflux membrane fusion protein, TriB
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.22
Kyte-Doolittle Hydrophobicity Value -0.241
Molecular Weight (kDa) 38.3
Isoelectric Point (pI) 9.12

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff E-value Percent Identity CARD Version
TriB TriB is a membrane protein that is fused to TriA and both are required for the triclosan efflux pump function of TriABC-OpmH in P. aeruginosa. ARO:3003680 protein homolog model Perfect 0.0 1.0 1.1.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 416 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000155 (512 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0157
Search term: triB

Transposon Insertions in PAO1

Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_32_A5
Genomic Position 178852
Position in ORF 398(1071)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_50_A2
Genomic Position 178501
Position in ORF 47(1071)
Reference 15805499
Type ISlacZ/hah insertion site
Mutant ID UWGC: 10866
Genomic Position 178758
Position in ORF 304(1071)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 45741
Genomic Position 179348
Position in ORF 894(1071)
Reference 14617778

Transposon Insertions in Orthologs

References

Identification and characterization of TriABC-OpmH, a triclosan efflux pump of Pseudomonas aeruginosa requiring two membrane fusion proteins.
Mima T, Joshi S, Gomez-Escalada M, Schweizer HP
J. Bacteriol. 2007 Nov;189(21):7600-9
PubMed ID: 17720796