Pseudomonas aeruginosa PAO1, PA0845 (cerN)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0845
Name
cerN
Replicon chromosome
Genomic location 921792 - 923804 (- strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_249536.1
GI 15596042
Affymetrix PA0845_at
Entrez 880698
GenBank AAG04234.1
NCBI Locus Tag PA0845
protein_id(GenBank) gb|AAG04234.1|AE004519_5|gnl|PseudoCAP|PA0845
TIGR NTL03PA00846
UniProtKB Acc Q9I596
UniProtKB ID NCASE_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
CerN
Product Name Confidence: Class 1
CommentNeutral ceramidase regulated by the sphingosine-sensing transcription factor SphR
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.76
Kyte-Doolittle Hydrophobicity Value -0.266
Molecular Weight (kDa) 73.4
Isoelectric Point (pI) 6.17

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2ZWS HYDROLASE 12/17/08 Crystal Structure Analysis of neutral ceramidase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.4 X-RAY DIFFRACTION 99.5
2ZXC HYDROLASE 12/22/08 seramidase complexed with C2 PSEUDOMONAS AERUGINOSA 2.2 X-RAY DIFFRACTION 99.5

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
N-[(1R,2R,3E)-2-hydroxy-1-(hydroxymethyl)heptadec-3-en-1-yl]acetamide DB06960 DrugBank 5.0.6 Q9I596 Neutral ceramidase 100.0 670 0.0e+00

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 41 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000812 (303 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0845
Search term: cerN
Search term: CerN

Human Homologs

Ensembl 86, assembly GRCh38.p7
N-acylsphingosine amidohydrolase 2 [Source:HGNC Symbol;Acc:HGNC:18860]
E-value: 6.2e-117
Percent Identity: 35.8

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 10265
Genomic Position 922765
Position in ORF 974(2013)
Reference 14617778
Type ISphoA/hah insertion site
Mutant ID UWGC: 34666
Genomic Position 923129
Position in ORF 1338(2013)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_25_A10
Genomic Position 922415
Position in ORF 624(2013)
Reference 15805499

Transposon Insertions in Orthologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Detection of host-derived sphingosine by Pseudomonas aeruginosa is important for survival in the murine lung.
LaBauve AE, Wargo MJ
PLoS Pathog. 2014 Jan;10(1):e1003889
PubMed ID: 24465209
Molecular cloning, sequencing, and expression of the gene encoding alkaline ceramidase from Pseudomonas aeruginosa. Cloning of a ceramidase homologue from Mycobacterium tuberculosis.
Okino N, Ichinose S, Omori A, Imayama S, Nakamura T, Ito M
J. Biol. Chem. 1999 Dec;274(51):36616-22
PubMed ID: 10593963
Purification and characterization of a novel ceramidase from Pseudomonas aeruginosa.
Okino N, Tani M, Imayama S, Ito M
J. Biol. Chem. 1998 Jun;273(23):14368-73
PubMed ID: 9603946