Pseudomonas aeruginosa PAO1, PA2011 (liuE)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: gnyL
Synonym: mvaB
Replicon chromosome
Genomic location 2199762 - 2200664 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_250701.1
GI 15597207
Affymetrix PA2011_at
DNASU PaCD00006898
Entrez 878857
GenBank AAG05399.1
INSDC AAG05399.1
NCBI Locus Tag PA2011
protein_id(GenBank) gb|AAG05399.1|AE004627_7|gnl|PseudoCAP|PA2011
UniParc UPI00000D429D
UniProtKB Acc Q9I2A0
UniRef100 UniRef100_Q9I2A0
UniRef50 UniRef50_Q9I2A0
UniRef90 UniRef90_Q9I2A0


Feature Type CDS
Coding Frame 1
Product Name
3-hydroxy-3-methylglutaryl-CoA lyase
Product Name Confidence: Class 1
Synonyms Hydroxymethylglutaryl-coenzymeA lyase, GnyH
HMG-CoA lyase
HMG lyase, MvaB
3-hydroxy-gamma-carboxygeranoyl-CoA lyase, GnyL
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.98
Kyte-Doolittle Hydrophobicity Value 0.059
Molecular Weight (kDa) 31.8
Isoelectric Point (pI) 6.04

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2FTP LYASE 01/24/06 Crystal Structure of hydroxymethylglutaryl-CoA lyase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.4 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 338 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003428 (551 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA2011
Search term: liuE

Human Homologs

Ensembl 94, assembly GRCh38.p12
3-hydroxy-3-methylglutaryl-CoA lyase [Source:HGNC Symbol;Acc:HGNC:5005]
E-value: 9.0e-98
Percent Identity: 57.7


The gnyRDBHAL cluster is involved in acyclic isoprenoid degradation in Pseudomonas aeruginosa.
Díaz-Pérez AL, Zavala-Hernández AN, Cervantes C, Campos-García J
Appl. Environ. Microbiol. 2004 Sep;70(9):5102-10
PubMed ID: 15345388
The atu and liu clusters are involved in the catabolic pathways for acyclic monoterpenes and leucine in Pseudomonas aeruginosa.
Aguilar JA, Zavala AN, Díaz-Pérez C, Cervantes C, Díaz-Pérez AL, Campos-García J
Appl. Environ. Microbiol. 2006 Mar;72(3):2070-9
PubMed ID: 16517656
Methylcrotonyl-CoA and geranyl-CoA carboxylases are involved in leucine/isovalerate utilization (Liu) and acyclic terpene utilization (Atu), and are encoded by liuB/liuD and atuC/atuF, in Pseudomonas aeruginosa.
Höschle B, Gnau V, Jendrossek D
Microbiology (Reading, Engl.) 2005 Nov;151(Pt 11):3649-56
PubMed ID: 16272386
Identification of genes and proteins necessary for catabolism of acyclic terpenes and leucine/isovalerate in Pseudomonas aeruginosa.
Förster-Fromme K, Höschle B, Mack C, Bott M, Armbruster W, Jendrossek D
Appl. Environ. Microbiol. 2006 Jul;72(7):4819-28
PubMed ID: 16820476
Nucleotide sequence and expression in Escherichia coli of the 3-hydroxy-3-methylglutaryl coenzyme A lyase gene of Pseudomonas mevalonii.
Anderson DH, Rodwell VW
J. Bacteriol. 1989 Dec;171(12):6468-72
PubMed ID: 2687236
3-Hydroxy-3-methylglutaryl coenzyme A lyase: affinity labeling of the Pseudomonas mevalonii enzyme and assignment of cysteine-237 to the active site.
Hruz PW, Narasimhan C, Miziorko HM
Biochemistry 1992 Jul;31(29):6842-7
PubMed ID: 1637819
The Pseudomonas aeruginosa liuE gene encodes the 3-hydroxy-3-methylglutaryl coenzyme A lyase, involved in leucine and acyclic terpene catabolism.
Chávez-Avilés M, Díaz-Pérez AL, Reyes-de la Cruz H, Campos-García J
FEMS Microbiol. Lett. 2009 Jul;296(1):117-23
PubMed ID: 19459965