Pseudomonas aeruginosa PAO1, PA2321 (gntK)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA2321
Name
gntK
Synonym: gntV
Synonym: gnuK
Synonym: gluconokinase
Replicon chromosome
Genomic location 2560144 - 2560665 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_251011.1
GI 15597517
Affymetrix PA2321_at
DNASU PaCD00007021
Entrez 881898
GenBank AAG05709.1
INSDC AAG05709.1
NCBI Locus Tag PA2321
protein_id(GenBank) gb|AAG05709.1|AE004658_3|gnl|PseudoCAP|PA2321
TIGR NTL03PA02321
UniParc UPI00000D4262
UniProtKB Acc G3XD53
UniProtKB ID G3XD53_PSEAE
UniRef100 UniRef100_W0YTZ0
UniRef50 UniRef50_Q4K7W9
UniRef90 UniRef90_Q02MB7

Product

Feature Type CDS
Coding Frame 1
Product Name
GntK
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.31
Kyte-Doolittle Hydrophobicity Value 0.083
Molecular Weight (kDa) 18.5
Isoelectric Point (pI) 7.11

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 197 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003186 (542 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2321
Search term: gntK
Search term: GntK

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning and molecular genetic characterization of the Escherichia coli gntR, gntK, and gntU genes of GntI, the main system for gluconate metabolism.
Tong S, Porco A, Isturiz T, Conway T
J. Bacteriol. 1996 Jun;178(11):3260-9
PubMed ID: 8655507
Gene organization and transcriptional regulation of the gntRKU operon involved in gluconate uptake and catabolism of Escherichia coli.
Izu H, Adachi O, Yamada M
J. Mol. Biol. 1997 Apr;267(4):778-93
PubMed ID: 9135111
Identification of GntR as regulator of the glucose metabolism in Pseudomonas aeruginosa.
Daddaoua A, Corral-Lugo A, Ramos JL, Krell T.
Environ Microbiol. 2017 Sep;19(9):3721-3733.
PubMed ID: 28752954