Pseudomonas aeruginosa PAO1, PA3186 (oprB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA3186
Name
oprB
Replicon chromosome
Genomic location 3575912 - 3577276 (- strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs

Cross-References

RefSeq NP_251876.1
GI 15598382
Affymetrix PA3186_oprB_s_at
Entrez 882788
GenBank AAG06574.1
INSDC AAG06574.1
NCBI Locus Tag PA3186
protein_id(GenBank) gb|AAG06574.1|AE004742_10|gnl|PseudoCAP|PA3186
TIGR NTL03PA03186
UniParc UPI0000131F64
UniProtKB Acc Q51485
UniProtKB ID PORB_PSEAE
UniRef100 UniRef100_Q51485
UniRef50 UniRef50_Q51485
UniRef90 UniRef90_Q51485

Product

Feature Type CDS
Coding Frame 1
Product Name
Glucose/carbohydrate outer membrane porin OprB precursor
Product Name Confidence: Class 1
Synonyms D1
carbohydrate-selective porin OprB
Evidence for Translation
Detected using LC-MS/MS (50.75 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.16
Kyte-Doolittle Hydrophobicity Value -0.537
Molecular Weight (kDa) 50.8
Isoelectric Point (pI) 5.48

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 1
Outer Membrane Vesicle Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 51 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002430 (722 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3186
Search term: oprB

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning and nucleotide sequence of the Pseudomonas aeruginosa glucose-selective OprB porin gene and distribution of OprB within the family Pseudomonadaceae.
Wylie JL, Worobec EA
Eur. J. Biochem. 1994 Mar;220(2):505-12
PubMed ID: 8125108
The OprB porin plays a central role in carbohydrate uptake in Pseudomonas aeruginosa.
Wylie JL, Worobec EA
J. Bacteriol. 1995 Jun;177(11):3021-6
PubMed ID: 7768797
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344