Pseudomonas aeruginosa UCBPP-PA14, PA14_18970 (pldA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_18970
Name
pldA
Synonym: tle5
Synonym: type VI secretion lipase effector Tle5
Replicon chromosome
Genomic location 1635662 - 1638973 (- strand)
Transposon Mutants 2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq YP_789660.1
GI 116051504
Entrez 4381470
INSDC ABJ12740.1
NCBI Locus Tag PA14_18970
UniParc UPI00003960CE
UniProtKB Acc Q02QX6
UniProtKB ID Q02QX6_PSEAB
UniRef100 UniRef100_Q02QX6
UniRef50 UniRef50_Q9HYC2
UniRef90 UniRef90_Q9HYC2

Product

Feature Type CDS
Coding Frame 1
Product Name
phospholipase D
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -4.85
Kyte-Doolittle Hydrophobicity Value -0.312
Molecular Weight (kDa) 122.7
Isoelectric Point (pI) 6.60

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 18 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002156 (80 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_18970
Search term: pldA
Search term: phospholipase D

Human Homologs

Ensembl 86, assembly GRCh38.p7
phospholipase D2 [Source:HGNC Symbol;Acc:HGNC:9068]
E-value: 3.1e-25
Percent Identity: 32.3

References

Genetic and biochemical analyses of a eukaryotic-like phospholipase D of Pseudomonas aeruginosa suggest horizontal acquisition and a role for persistence in a chronic pulmonary infection model.
Wilderman PJ, Vasil AI, Johnson Z, Vasil ML
Mol. Microbiol. 2001 Jan;39(2):291-303
PubMed ID: 11136451
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A.
Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):7707-7712.
PubMed ID: 28673999
Biochemical characterization of a Pseudomonas aeruginosa phospholipase D.
Spencer C, Brown HA.
Biochemistry. 2015 Feb 10;54(5):1208-18.
PubMed ID: 25565226