Pseudomonas aeruginosa UCBPP-PA14, PA14_18970 (pldA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006)
GCF_000014625.1|latest
Locus Tag
PA14_18970
Name
pldA
Synonym: tle5
Synonym: type VI secretion lipase effector Tle5
Replicon chromosome
Genomic location 1635662 - 1638973 (- strand)
Transposon Mutants 2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq YP_789660.1
GI 116051504
Entrez 4381470
INSDC ABJ12740.1
NCBI Locus Tag PA14_18970
UniParc UPI00003960CE
UniProtKB Acc Q02QX6
UniProtKB ID Q02QX6_PSEAB
UniRef100 UniRef100_Q02QX6
UniRef50 UniRef50_Q9HYC2
UniRef90 UniRef90_Q9HYC2

Product

Feature Type CDS
Coding Frame 1
Product Name
phospholipase D
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -4.85
Kyte-Doolittle Hydrophobicity Value -0.312
Molecular Weight (kDa) 122.7
Isoelectric Point (pI) 6.60

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7V53 HYDROLASE 08/16/21 Crystal structure of full-length phospholipase D from Pseudomonas aeruginosa PAO1 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.1 X-RAY DIFFRACTION 98.7
7WDK IMMUNE SYSTEM 12/21/21 The structure of PldA-PA3488 complex Pseudomonas aeruginosa; Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.05 ELECTRON MICROSCOPY 98.7
7V55 HYDROLASE 08/16/21 Crystal structure of phospholipase D from Pseudomonas aeruginosa PAO1 using in situ proteolysis Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3 X-RAY DIFFRACTION 98.7

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 18 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002156 (79 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_18970
Search term: pldA
Search term: phospholipase D

Human Homologs

Ensembl 110, assembly GRCh38.p14
phospholipase D1 [Source:HGNC Symbol;Acc:HGNC:9067]
E-value: 8.0e-26
Percent Identity: 30.7
Ensembl 110, assembly GRCh38.p14
phospholipase D1 [Source:HGNC Symbol;Acc:HGNC:9067]
E-value: 8.0e-26
Percent Identity: 30.7

References

Genetic and biochemical analyses of a eukaryotic-like phospholipase D of Pseudomonas aeruginosa suggest horizontal acquisition and a role for persistence in a chronic pulmonary infection model.
Wilderman PJ, Vasil AI, Johnson Z, Vasil ML
Mol Microbiol 2001 Jan;39(2):291-303
PubMed ID: 11136451
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A
Proc Natl Acad Sci U S A 2017 Jul 18;114(29):7707-7712
PubMed ID: 28673999
Biochemical characterization of a Pseudomonas aeruginosa phospholipase D.
Spencer C, Brown HA
Biochemistry 2015 Feb 10;54(5):1208-18
PubMed ID: 25565226