Pseudomonas aeruginosa UCBPP-PA14, PA14_19010 (tsi3)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_19010
Name
tsi3
Replicon chromosome
Genomic location 1641698 - 1642135 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq YP_789662.1
GI 116051503
Entrez 4381469
INSDC ABJ12739.1
NCBI Locus Tag PA14_19010
UniParc UPI00003960CB
UniProtKB Acc Q02QX4
UniProtKB ID Q02QX4_PSEAB
UniRef100 UniRef100_Q02QX4
UniRef50 UniRef50_A6V1U0
UniRef90 UniRef90_Q02QX4

Product

Feature Type CDS
Coding Frame 1
Product Name
Tsi3
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) -3.79
Kyte-Doolittle Hydrophobicity Value -0.450
Molecular Weight (kDa) 15.8
Isoelectric Point (pI) 4.85

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4M5F HYDROLASE INHIBITOR 08/08/13 complex structure of Tse3-Tsi3 PSEUDOMONAS AERUGINOSA 2.5 X-RAY DIFFRACTION 99.2
4LUQ PROTEIN BINDING/TOXIN INHIBITOR 07/25/13 Crystal structure of virulence effector Tse3 in complex with neutralizer Tsi3 PSEUDOMONAS AERUGINOSA 1.77 X-RAY DIFFRACTION 97.6
4N88 HYDROLASE/HYDROLASE INHIBITOR 10/17/13 Crystal structure of Tse3-Tsi3 complex with calcium ion PSEUDOMONAS AERUGINOSA 2.8 X-RAY DIFFRACTION 99.2
4N80 HYDROLASE/HYDROLASE INHIBITOR 10/16/13 Crystal structure of Tse3-Tsi3 complex PSEUDOMONAS AERUGINOSA 2.4 X-RAY DIFFRACTION 97.6
4N7S HYDROLASE/HYDROLASE INHIBITOR 10/16/13 Crystal structure of Tse3-Tsi3 complex with Zinc ion PSEUDOMONAS AERUGINOSA 2.101 X-RAY DIFFRACTION 99.2
3WA5 HYDROLASE 04/26/13 Crystal Structure of type VI peptidoglycan muramidase effector Tse3 in complex with its cognate immunity protein Tsi3 PSEUDOMONAS AERUGINOSA 1.9 X-RAY DIFFRACTION 98.6

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 2 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002157 (303 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_19010
Search term: tsi3
Search term: Tsi3

References

RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
Allsopp LP, Wood TE, Howard SA, Maggiorelli F, Nolan LM, Wettstadt S, Filloux A.
Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):7707-7712.
PubMed ID: 28673999
Structural insights into the T6SS effector protein Tse3 and the Tse3-Tsi3 complex from Pseudomonas aeruginosa reveal a calcium-dependent membrane-binding mechanism.
Lu D, Shang G, Zhang H, Yu Q, Cong X, Yuan J, He F, Zhu C, Zhao Y, Yin K, Chen Y, Hu J, Zhang X, Yuan Z, Xu S, Hu W, Cang H, Gu L.
Mol Microbiol. 2014 Jun;92(5):1092-112.
PubMed ID: 24724564