Pseudomonas aeruginosa PAO1, PA3975 (thiD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3975
Name
thiD
Replicon chromosome
Genomic location 4455887 - 4456684 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252664.1
GI 15599170
Affymetrix PA3975_thiD_at
DNASU PaCD00007736
Entrez 878891
GenBank AAG07362.1
INSDC AAG07362.1
NCBI Locus Tag PA3975
protein_id(GenBank) gb|AAG07362.1|AE004815_6|gnl|PseudoCAP|PA3975
TIGR NTL03PA03975
UniParc UPI00000C5BAB
UniProtKB Acc Q9HX41
UniProtKB ID Q9HX41_PSEAE
UniRef100 UniRef100_Q9HX41
UniRef50 UniRef50_Q7P1R2
UniRef90 UniRef90_Q9HX41

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphomethylpyrimidine kinase
Product Name Confidence: Class 2
Synonyms HMP-P kinase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.36
Kyte-Doolittle Hydrophobicity Value -0.017
Molecular Weight (kDa) 28.0
Isoelectric Point (pI) 6.22

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 564 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001706 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3975
Search term: thiD

Human Homologs

References

Identification and characterization of an operon in Salmonella typhimurium involved in thiamine biosynthesis.
Petersen LA, Downs DM
J Bacteriol 1997 Aug;179(15):4894-900
PubMed ID: 9244280