Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA4930
|
Name |
alr
|
Replicon | chromosome |
Genomic location | 5533017 - 5534093 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 6 transposon mutants in orthologs |
Comment |
RefSeq | NP_253617.1 |
GI | 15600123 |
Affymetrix | PA4930_alr_at |
DNASU | PaCD00008135 |
Entrez | 878646 |
GenBank | AAG08315.1 |
INSDC | AAG08315.1 |
NCBI Locus Tag | PA4930 |
protein_id(GenBank) | gb|AAG08315.1|AE004906_3|gnl|PseudoCAP|PA4930 |
TIGR | NTL03PA04931 |
UniParc | UPI00001258C3 |
UniProtKB Acc | Q9HUN4 |
UniProtKB ID | ALR1_PSEAE |
UniRef100 | UniRef100_Q9HUN4 |
UniRef50 | UniRef50_Q9HUN4 |
UniRef90 | UniRef90_Q9HUN4 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
biosynthetic alanine racemase
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -5.53 |
Kyte-Doolittle Hydrophobicity Value | 0.128 |
Molecular Weight (kDa) | 38.3 |
Isoelectric Point (pI) | 6.13 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6A2F | ISOMERASE | 06/11/18 | Crystal structure of biosynthetic alanine racemase from Pseudomonas aeruginosa | Pseudomonas aeruginosa PAO1 | 2.5 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 558 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004545 (850 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA4930
Search term: alr
Search term: biosynthetic alanine racemase
|
Biosynthetic alr alanine racemase from Salmonella typhimurium: DNA and protein sequence determination.
Galakatos NG, Daub E, Botstein D, Walsh CT
Biochemistry 1986 Jun 3;25(11):3255-60
PubMed ID: 3524676
|
Correlation between catalytic activity and monomer-dimer equilibrium of bacterial alanine racemases.
Ju J, Xu S, Furukawa Y, Zhang Y, Misono H, Minamino T, Namba K, Zhao B, Ohnishi K
J Biochem 2011 Jan;149(1):83-9
PubMed ID: 20971724
|
Enzymatic characterization and crystal structure of biosynthetic alanine racemase from Pseudomonas aeruginosa PAO1.
Dong H, Han Q, Guo Y, Ju J, Wang S, Yuan C, Long W, He X, Xu S, Li S
Biochem Biophys Res Commun 2018 Sep 18;503(4):2319-2325
PubMed ID: 29964014
|