Pseudomonas aeruginosa PAO1, PA4944 (hfq)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4944
Name
hfq
Synonym: hfq
Replicon chromosome
Genomic location 5548397 - 5548645 (- strand)

Cross-References

RefSeq NP_253631.1
GI 15600137
Affymetrix PA4944_at
Entrez 878015
GenBank AAG08329.1
INSDC AAG08329.1
NCBI Locus Tag PA4944
protein_id(GenBank) gb|AAG08329.1|AE004907_7|gnl|PseudoCAP|PA4944
TIGR NTL03PA04945
UniParc UPI000012C6A3
UniProtKB Acc Q9HUM0
UniProtKB ID HFQ_PSEAE
UniRef100 UniRef100_A6VD57
UniRef50 UniRef50_Q9ZI76
UniRef90 UniRef90_Q4KJ73

Product

Feature Type CDS
Coding Frame 1
Product Name
Hfq
Product Name Confidence: Class 1
Synonyms Hfq
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 3.46
Kyte-Doolittle Hydrophobicity Value -0.244
Molecular Weight (kDa) 9.1
Isoelectric Point (pI) 10.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1U1T RNA BINDING PROTEIN 07/16/04 Hfq protein from Pseudomonas aeruginosa. High-salt crystals PSEUDOMONAS AERUGINOSA 1.9 X-RAY DIFFRACTION 100.0
4J6Y RNA BINDING PROTEIN 02/12/13 Hfq from Pseudomonas aeruginosa crystallized in GTP presence PSEUDOMONAS AERUGINOSA 2.1405 X-RAY DIFFRACTION 100.0
1U1S RNA BINDING PROTEIN 07/16/04 Hfq protein from Pseudomonas aeruginosa. Low-salt crystals PSEUDOMONAS AERUGINOSA 1.6 X-RAY DIFFRACTION 100.0
4J5Y RNA BINDING PROTEIN 02/11/13 Crystal structure of Hfq from Pseudomonas aeruginosa in complex with ATP PSEUDOMONAS AERUGINOSA 2.0953 X-RAY DIFFRACTION 100.0
4J6W RNA BINDING PROTEIN 02/12/13 Crystal structure of HFQ from Pseudomonas aeruginosa in complex with CTP PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 100.0
4J6X RNA BINDING PROTEIN 02/12/13 Crystal structure of Hfq from Pseudomonas aeruginosa in complex with UTP PSEUDOMONAS AERUGINOSA 2.222 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database Victors : 4214
Category
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Mus musculus
Notes
Reference 14521880
Source 2
Database Victors : 4214
Category
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Galleria mellonella
Notes
Reference 14521880

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 282 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004559 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4944
Search term: hfq
Search term: Hfq

References

Hfq-dependent alterations of the transcriptome profile and effects on quorum sensing in Pseudomonas aeruginosa.
Sonnleitner E, Schuster M, Sorger-Domenigg T, Greenberg EP, Bläsi U
Mol. Microbiol. 2006 Mar;59(5):1542-58
PubMed ID: 16468994
Reduced virulence of a hfq mutant of Pseudomonas aeruginosa O1.
Sonnleitner E, Hagens S, Rosenau F, Wilhelm S, Habel A, Jäger KE, Bläsi U
Microb. Pathog. 2003 Nov;35(5):217-28
PubMed ID: 14521880
yst gene expression in Yersinia enterocolitica is positively regulated by a chromosomal region that is highly homologous to Escherichia coli host factor 1 gene (hfq).
Nakao H, Watanabe H, Nakayama S, Takeda T
Mol. Microbiol. 1995 Dec;18(5):859-65
PubMed ID: 8825090
DNA binding properties of the hfq gene product of Escherichia coli.
Takada A, Wachi M, Kaidow A, Takamura M, Nagai K
Biochem. Biophys. Res. Commun. 1997 Jul;236(3):576-9
PubMed ID: 9245691