Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5243
|
Name |
hemB
|
Replicon | chromosome |
Genomic location | 5904614 - 5905627 (- strand) |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_253930.1 |
GI | 15600436 |
Affymetrix | PA5243_hemB_at |
DNASU | PaCD00008258 |
Entrez | 879701 |
GenBank | AAG08628.1 |
INSDC | AAG08628.1 |
NCBI Locus Tag | PA5243 |
protein_id(GenBank) | gb|AAG08628.1|AE004936_11|gnl|PseudoCAP|PA5243 |
TIGR | NTL03PA05244 |
UniParc | UPI000011076B |
UniProtKB Acc | Q59643 |
UniProtKB ID | HEM2_PSEAE |
UniRef100 | UniRef100_Q59643 |
UniRef50 | UniRef50_Q59643 |
UniRef90 | UniRef90_Q59643 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
delta-aminolevulinic acid dehydratase
|
Synonyms |
porphobilinogen synthase |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -9.79 |
Kyte-Doolittle Hydrophobicity Value | -0.166 |
Molecular Weight (kDa) | 37.0 |
Isoelectric Point (pI) | 4.83 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
1W5M | SYNTHASE | 08/09/04 | Stepwise introduction of zinc binding site into porphobilinogen synthase of Pseudomonas aeruginosa (mutations A129C and D139C) | PSEUDOMONAS AERUGINOSA | 1.6 | X-RAY DIFFRACTION | 99.1 |
2C13 | LYASE | 09/11/05 | 5-hydroxy-levulinic acid bound to Porphobilinogen synthase from Pseudomonas aeruginosa | PSEUDOMONAS AERUGINOSA | 2.15 | X-RAY DIFFRACTION | 99.7 |
1W56 | SYNTHASE | 08/05/04 | Stepwise introduction of zinc binding site into porphobilinogen synthase of Pseudomonas aeruginosa (mutations A129C and D131C) | PSEUDOMONAS AERUGINOSA | 1.7 | X-RAY DIFFRACTION | 99.1 |
2WOQ | LYASE/ANTIBIOTIC | 07/27/09 | Porphobilinogen Synthase (HemB) in Complex with 5-acetamido-4- oxohexanoic acid (Alaremycin 2) | PSEUDOMONAS AERUGINOSA | 1.75 | X-RAY DIFFRACTION | 100.0 |
2C16 | LYASE | 09/11/05 | 5-(4-Carboxy-2-oxo-butane-1-sulfinyl)-4-oxo-pentanoic acid acid bound to Porphobilinogen synthase from Pseudomonas aeruginosa | PSEUDOMONAS AERUGINOSA | 2.02 | X-RAY DIFFRACTION | 99.7 |
1B4K | LYASE | 12/22/98 | High resolution crystal structure of a MG2-dependent 5-aminolevulinic acid dehydratase | Pseudomonas aeruginosa | 1.67 | X-RAY DIFFRACTION | 100.0 |
2C19 | LYASE | 09/11/05 | 5-(4-Carboxy-2-oxo-butylsulfanyl)-4-oxo-pentanoic acid acid bound to Porphobilinogen synthase from Pseudomonas aeruginosa | PSEUDOMONAS AERUGINOSA | 2.05 | X-RAY DIFFRACTION | 99.7 |
1GZG | LYASE | 05/21/02 | Complex of a Mg2-dependent porphobilinogen synthase from Pseudomonas aeruginosa (mutant D139N) with 5-fluorolevulinic acid | PSEUDOMONAS AERUGINOSA | 1.66 | X-RAY DIFFRACTION | 99.4 |
2C18 | LYASE | 09/11/05 | 5-(4-Carboxy-2-oxo-butane-1-sulfonyl)-4-oxo-pentanoic acid bound to Porphobilinogen synthase from Pseudomonas aeruginosa | PSEUDOMONAS AERUGINOSA | 1.93 | X-RAY DIFFRACTION | 99.7 |
2C14 | LYASE | 09/11/05 | 5-(4-Carboxy-2-oxo-butylamino)-4-oxo-pentanoic acid acid bound to Porphobilinogen synthase from Pseudomonas aeruginosa | PSEUDOMONAS AERUGINOSA | 1.9 | X-RAY DIFFRACTION | 99.7 |
1W5N | SYNTHASE | 08/09/04 | Stepwise introduction of zinc binding site into porphobilinogen synthase of Pseudomonas aeruginosa (mutations D131C and D139C) | PSEUDOMONAS AERUGINOSA | 1.65 | X-RAY DIFFRACTION | 99.1 |
2C15 | LYASE | 09/11/05 | 5-(4-Carboxy-2-oxo-butoxy)-4-oxo-pentanoic acid acid bound to Porphobilinogen synthase from Pseudomonas aeruginosa | PSEUDOMONAS AERUGINOSA | 1.48 | X-RAY DIFFRACTION | 99.7 |
1W54 | SYNTHASE | 08/05/04 | Stepwise introduction of a zinc binding site into Porphobilinogen synthase from Pseudomonas aeruginosa (mutation D139C) | PSEUDOMONAS AERUGINOSA | 2.2 | X-RAY DIFFRACTION | 99.4 |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
Formic acid | DB01942 | DrugBank | 5.1.4 | Q59643 | Delta-aminolevulinic acid dehydratase | 100.0 | 337 | 6.3e-187 |
5-Fluorolevulinic Acid | DB02828 | DrugBank | 5.1.4 | Q59643 | Delta-aminolevulinic acid dehydratase | 100.0 | 337 | 6.3e-187 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 582 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004823 (536 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5243
Search term: hemB
Search term: delta-aminolevulinic acid dehydratase
|
Ensembl
110, assembly
GRCh38.p14
|
aminolevulinate dehydratase [Source:HGNC Symbol;Acc:HGNC:395]
E-value:
4.6e-57
Percent Identity:
44.3
|
Cloning, mapping and functional characterization of the hemB gene of Pseudomonas aeruginosa, which encodes a magnesium-dependent 5-aminolevulinic acid dehydratase.
Frankenberg N, Kittel T, Hungerer C, Römling U, Jahn D
Mol Gen Genet 1998 Feb;257(4):485-9
PubMed ID: 9529530
|
Production, purification, and characterization of a Mg2+-responsive porphobilinogen synthase from Pseudomonas aeruginosa.
Frankenberg N, Heinz DW, Jahn D
Biochemistry 1999 Oct 19;38(42):13968-75
PubMed ID: 10529243
|
Pseudomonas aeruginosa contains a novel type V porphobilinogen synthase with no required catalytic metal ions.
Frankenberg N, Jahn D, Jaffe EK
Biochemistry 1999 Oct 19;38(42):13976-82
PubMed ID: 10529244
|