Pseudomonas aeruginosa PAO1, PA5243 (hemB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5243
Name
hemB
Replicon chromosome
Genomic location 5904614 - 5905627 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253930.1
GI 15600436
Affymetrix PA5243_hemB_at
DNASU PaCD00008258
Entrez 879701
GenBank AAG08628.1
INSDC AAG08628.1
NCBI Locus Tag PA5243
protein_id(GenBank) gb|AAG08628.1|AE004936_11|gnl|PseudoCAP|PA5243
TIGR NTL03PA05244
UniParc UPI000011076B
UniProtKB Acc Q59643
UniProtKB ID HEM2_PSEAE
UniRef100 UniRef100_Q59643
UniRef50 UniRef50_Q59643
UniRef90 UniRef90_Q59643

Product

Feature Type CDS
Coding Frame 1
Product Name
delta-aminolevulinic acid dehydratase
Product Name Confidence: Class 1
Synonyms porphobilinogen synthase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.79
Kyte-Doolittle Hydrophobicity Value -0.166
Molecular Weight (kDa) 37.0
Isoelectric Point (pI) 4.83

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1W5M SYNTHASE 08/09/04 Stepwise introduction of zinc binding site into porphobilinogen synthase of Pseudomonas aeruginosa (mutations A129C and D139C) PSEUDOMONAS AERUGINOSA 1.6 X-RAY DIFFRACTION 99.1
2C13 LYASE 09/11/05 5-hydroxy-levulinic acid bound to Porphobilinogen synthase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.15 X-RAY DIFFRACTION 99.7
1W56 SYNTHASE 08/05/04 Stepwise introduction of zinc binding site into porphobilinogen synthase of Pseudomonas aeruginosa (mutations A129C and D131C) PSEUDOMONAS AERUGINOSA 1.7 X-RAY DIFFRACTION 99.1
2WOQ LYASE/ANTIBIOTIC 07/27/09 Porphobilinogen Synthase (HemB) in Complex with 5-acetamido-4- oxohexanoic acid (Alaremycin 2) PSEUDOMONAS AERUGINOSA 1.75 X-RAY DIFFRACTION 100.0
2C16 LYASE 09/11/05 5-(4-Carboxy-2-oxo-butane-1-sulfinyl)-4-oxo-pentanoic acid acid bound to Porphobilinogen synthase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.02 X-RAY DIFFRACTION 99.7
1B4K LYASE 12/22/98 High resolution crystal structure of a MG2-dependent 5-aminolevulinic acid dehydratase Pseudomonas aeruginosa 1.67 X-RAY DIFFRACTION 100.0
2C19 LYASE 09/11/05 5-(4-Carboxy-2-oxo-butylsulfanyl)-4-oxo-pentanoic acid acid bound to Porphobilinogen synthase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.05 X-RAY DIFFRACTION 99.7
1GZG LYASE 05/21/02 Complex of a Mg2-dependent porphobilinogen synthase from Pseudomonas aeruginosa (mutant D139N) with 5-fluorolevulinic acid PSEUDOMONAS AERUGINOSA 1.66 X-RAY DIFFRACTION 99.4
2C18 LYASE 09/11/05 5-(4-Carboxy-2-oxo-butane-1-sulfonyl)-4-oxo-pentanoic acid bound to Porphobilinogen synthase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.93 X-RAY DIFFRACTION 99.7
2C14 LYASE 09/11/05 5-(4-Carboxy-2-oxo-butylamino)-4-oxo-pentanoic acid acid bound to Porphobilinogen synthase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.9 X-RAY DIFFRACTION 99.7
1W5N SYNTHASE 08/09/04 Stepwise introduction of zinc binding site into porphobilinogen synthase of Pseudomonas aeruginosa (mutations D131C and D139C) PSEUDOMONAS AERUGINOSA 1.65 X-RAY DIFFRACTION 99.1
2C15 LYASE 09/11/05 5-(4-Carboxy-2-oxo-butoxy)-4-oxo-pentanoic acid acid bound to Porphobilinogen synthase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.48 X-RAY DIFFRACTION 99.7
1W54 SYNTHASE 08/05/04 Stepwise introduction of a zinc binding site into Porphobilinogen synthase from Pseudomonas aeruginosa (mutation D139C) PSEUDOMONAS AERUGINOSA 2.2 X-RAY DIFFRACTION 99.4

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Formic acid DB01942 DrugBank 5.1.4 Q59643 Delta-aminolevulinic acid dehydratase 100.0 337 6.3e-187
5-Fluorolevulinic Acid DB02828 DrugBank 5.1.4 Q59643 Delta-aminolevulinic acid dehydratase 100.0 337 6.3e-187

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 582 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004823 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5243
Search term: hemB

Human Homologs

Ensembl 110, assembly GRCh38.p14
aminolevulinate dehydratase [Source:HGNC Symbol;Acc:HGNC:395]
E-value: 4.6e-57
Percent Identity: 44.3

References

Cloning, mapping and functional characterization of the hemB gene of Pseudomonas aeruginosa, which encodes a magnesium-dependent 5-aminolevulinic acid dehydratase.
Frankenberg N, Kittel T, Hungerer C, Römling U, Jahn D
Mol Gen Genet 1998 Feb;257(4):485-9
PubMed ID: 9529530
Production, purification, and characterization of a Mg2+-responsive porphobilinogen synthase from Pseudomonas aeruginosa.
Frankenberg N, Heinz DW, Jahn D
Biochemistry 1999 Oct 19;38(42):13968-75
PubMed ID: 10529243
Pseudomonas aeruginosa contains a novel type V porphobilinogen synthase with no required catalytic metal ions.
Frankenberg N, Jahn D, Jaffe EK
Biochemistry 1999 Oct 19;38(42):13976-82
PubMed ID: 10529244