Pseudomonas aeruginosa PAO1, PA4514

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4514
Name
Synonym: piuA
Replicon chromosome
Genomic location 5053616 - 5055877 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 13 transposon mutants in orthologs

Cross-References

RefSeq NP_253204.1
GI 15599710
Affymetrix PA4514_at
Entrez 881112
GenBank AAG07902.1
INSDC AAG07902.1
NCBI Locus Tag PA4514
protein_id(GenBank) gb|AAG07902.1|AE004865_3|gnl|PseudoCAP|PA4514
TIGR NTL03PA04515
UniParc UPI00000D4235
UniProtKB Acc G3XCY8
UniProtKB ID G3XCY8_PSEAE
UniRef100 UniRef100_O68590
UniRef50 UniRef50_O68590
UniRef90 UniRef90_O68590

Product

Feature Type CDS
Coding Frame 1
Product Name
probable outer membrane receptor for iron transport
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (82.34 kDa, PMID:21751344)
Charge (pH 7) -6.63
Kyte-Doolittle Hydrophobicity Value -0.594
Molecular Weight (kDa) 82.3
Isoelectric Point (pI) 5.90

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Vesicle Class 1
Outer Membrane Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5FOK METAL TRANSPORT 11/23/15 Crystal structure of the siderophore receptor PiuA from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.9 X-RAY DIFFRACTION 99.9

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 283 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000149 (2669 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4514

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
FhuA, a transporter of the Escherichia coli outer membrane, is converted into a channel upon binding of bacteriophage T5.
Bonhivers M, Ghazi A, Boulanger P, Letellier L
EMBO J 1996 Apr 15;15(8):1850-6
PubMed ID: 8617231
Gene repression by the ferric uptake regulator in Pseudomonas aeruginosa: cycle selection of iron-regulated genes.
Ochsner UA, Vasil ML
Proc Natl Acad Sci U S A 1996 Apr 30;93(9):4409-14
PubMed ID: 8633080
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344