Pseudomonas aeruginosa PAO1, PA5464

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5464
Name
Replicon chromosome
Genomic location 6152718 - 6154676 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_254151.1
GI 15600657
Affymetrix PA5464_at
Entrez 879368
GenBank AAG08849.1
INSDC AAG08849.1
NCBI Locus Tag PA5464
protein_id(GenBank) gb|AAG08849.1|AE004959_5|gnl|PseudoCAP|PA5464
TIGR NTL03PA05465
UniParc UPI00000C601D
UniProtKB Acc Q9HTA6
UniProtKB ID Q9HTA6_PSEAE
UniRef100 UniRef100_Q9HTA6
UniRef50 UniRef50_Q9HTA6
UniRef90 UniRef90_Q9HTA6

Product

Feature Type CDS
Coding Frame 1
Product Name
hypothetical protein
Product Name Confidence: Class 4
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -0.10
Kyte-Doolittle Hydrophobicity Value -0.540
Molecular Weight (kDa) 74.3
Isoelectric Point (pI) 6.99

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 15 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005008 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5464

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994