Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA1777
|
Name |
oprF
|
Replicon | chromosome |
Genomic location | 1921174 - 1922226 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
Comment | Localication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878). |
RefSeq | NP_250468.1 |
GI | 15596974 |
Affymetrix | PA1777_oprF_at |
Entrez | 878442 |
GenBank | AAG05166.1 |
INSDC | AAG05166.1 |
NCBI Locus Tag | PA1777 |
protein_id(GenBank) | gb|AAG05166.1|AE004603_6|gnl|PseudoCAP|PA1777 |
TIGR | NTL03PA01778 |
UniParc | UPI000002B99B |
UniProtKB Acc | P13794 |
UniProtKB ID | PORF_PSEAE |
UniRef100 | UniRef100_P13794 |
UniRef50 | UniRef50_P13794 |
UniRef90 | UniRef90_P13794 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
Major porin and structural outer membrane porin OprF precursor
|
Synonyms |
Porin F |
Evidence for Translation |
Detected by two-dimensional gel electrophoresis and protein mass mapping(PMID:21863142).
Detected using LC-MS/MS (37.64 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.0, molecular weight:37.6, PMID:21674802)
Detected using 2-DE, MALDI-TOF/MS analysis (37.64 kDa, pI 5.0, PMID:16335974)
|
Charge (pH 7) | -11.67 |
Kyte-Doolittle Hydrophobicity Value | -0.443 |
Molecular Weight (kDa) | 37.6 |
Isoelectric Point (pI) | 4.75 |
Individual Mappings | |
Additional evidence for subcellular localization |
Detected in sarcosine-insoluble outer membrane fraction using 2-DE, MALDI-TOF/MS analysis (PMID:16335974)
Detected in a periplasmic protein preparation by spheroplasting by lysozyme and sucrose.(PMID:19333994)
|
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
5U1H | PEPTIDE BINDING PROTEIN | 11/28/16 | Crystal structure of the C-terminal peptidoglycan binding domain of OprF (PA1777) from Pseudomonas aeruginosa | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.5 | X-RAY DIFFRACTION | 100.0 |
4RLC | TRANSPORT PROTEIN | 10/16/14 | Crystal structure of the N-terminal beta-barrel domain of Pseudomonas aeruginosa OprF | Pseudomonas aeruginosa | 1.6 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 441 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG003618 (537 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA1777
Search term: oprF
|
Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Sequence and transcriptional start site of the Pseudomonas aeruginosa outer membrane porin protein F gene.
Duchêne M, Schweizer A, Lottspeich F, Krauss G, Marget M, Vogel K, von Specht BU, Domdey H
J Bacteriol 1988 Jan;170(1):155-62
PubMed ID: 2447060
|
Full virulence of Pseudomonas aeruginosa requires OprF.
Fito-Boncompte L, Chapalain A, Bouffartigues E, Chaker H, Lesouhaitier O, Gicquel G, Bazire A, Madi A, Connil N, Véron W, Taupin L, Toussaint B, Cornelis P, Wei Q, Shioya K, Déziel E, Feuilloley MG, Orange N, Dufour A, Chevalier S
Infect Immun 2011 Mar;79(3):1176-86
PubMed ID: 21189321
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
|
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
|
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
|
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
|
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878
|