Pseudomonas aeruginosa PAO1, PA1777 (oprF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1777
Name
oprF
Replicon chromosome
Genomic location 1921174 - 1922226 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq NP_250468.1
GI 15596974
Affymetrix PA1777_oprF_at
Entrez 878442
GenBank AAG05166.1
INSDC AAG05166.1
NCBI Locus Tag PA1777
protein_id(GenBank) gb|AAG05166.1|AE004603_6|gnl|PseudoCAP|PA1777
TIGR NTL03PA01778
UniParc UPI000002B99B
UniProtKB Acc P13794
UniProtKB ID PORF_PSEAE
UniRef100 UniRef100_P13794
UniRef50 UniRef50_P13794
UniRef90 UniRef90_P13794

Product

Feature Type CDS
Coding Frame 1
Product Name
Major porin and structural outer membrane porin OprF precursor
Product Name Confidence: Class 1
Synonyms Porin F
Evidence for Translation
Detected by two-dimensional gel electrophoresis and protein mass mapping(PMID:21863142).
Detected using LC-MS/MS (37.64 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.0, molecular weight:37.6, PMID:21674802)
Detected using 2-DE, MALDI-TOF/MS analysis (37.64 kDa, pI 5.0, PMID:16335974)
Charge (pH 7) -11.67
Kyte-Doolittle Hydrophobicity Value -0.443
Molecular Weight (kDa) 37.6
Isoelectric Point (pI) 4.75

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Vesicle Class 1
Outer Membrane Class 1
Periplasmic Class 1
Additional evidence for subcellular localization
Detected in sarcosine-insoluble outer membrane fraction using 2-DE, MALDI-TOF/MS analysis (PMID:16335974)
Detected in a periplasmic protein preparation by spheroplasting by lysozyme and sucrose.(PMID:19333994)

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5U1H PEPTIDE BINDING PROTEIN 11/28/16 Crystal structure of the C-terminal peptidoglycan binding domain of OprF (PA1777) from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.5 X-RAY DIFFRACTION 100.0
4RLC TRANSPORT PROTEIN 10/16/14 Crystal structure of the N-terminal beta-barrel domain of Pseudomonas aeruginosa OprF Pseudomonas aeruginosa 1.6 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 441 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003618 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1777
Search term: oprF

Human Homologs

References

Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.
Peng X, Xu C, Ren H, Lin X, Wu L, Wang S
J Proteome Res 2005 Nov-Dec;4(6):2257-65
PubMed ID: 16335974
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Sequence and transcriptional start site of the Pseudomonas aeruginosa outer membrane porin protein F gene.
Duchêne M, Schweizer A, Lottspeich F, Krauss G, Marget M, Vogel K, von Specht BU, Domdey H
J Bacteriol 1988 Jan;170(1):155-62
PubMed ID: 2447060
Full virulence of Pseudomonas aeruginosa requires OprF.
Fito-Boncompte L, Chapalain A, Bouffartigues E, Chaker H, Lesouhaitier O, Gicquel G, Bazire A, Madi A, Connil N, Véron W, Taupin L, Toussaint B, Cornelis P, Wei Q, Shioya K, Déziel E, Feuilloley MG, Orange N, Dufour A, Chevalier S
Infect Immun 2011 Mar;79(3):1176-86
PubMed ID: 21189321
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878