Pseudomonas fluorescens SBW25, PFLU3300

Gene Feature Overview

Strain	Pseudomonas fluorescens SBW25 GCF_000009225.2|latest
Locus Tag	PFLU3300
Name
Replicon	chromosome
Genomic location	3653435 - 3654265 (- strand)

Cross-References

RefSeq	YP_002872871.1
GI	229590752
Entrez	7820068
INSDC	CAY49526.1
UniParc	UPI00019DA729
UniProtKB Acc	C3KCA0
UniProtKB ID	C3KCA0_PSEFS
UniRef100	UniRef100_V9QU79
UniRef50	UniRef50_O69762
UniRef90	UniRef90_O69762

Product

Feature Type	CDS
Coding Frame	1
Product Name	p-hydroxycinnamoyl CoA hydratase/lyase
Synonyms
Evidence for Translation
Charge (pH 7)	-3.63
Kyte-Doolittle Hydrophobicity Value	-0.347
Molecular Weight (kDa)	31.0
Isoelectric Point (pI)	5.28

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
2J5I	LYASE	09/18/06	Crystal Structure of Hydroxycinnamoyl-CoA Hydratase-Lyase	PSEUDOMONAS FLUORESCENS	1.8	X-RAY DIFFRACTION	98.6
2VSU	LYASE	04/29/08	A ternary complex of Hydroxycinnamoyl-CoA Hydratase-Lyase (HCHL) with acetyl-Coenzyme A and vanillin gives insights into substrate specificity and mechanism.	PSEUDOMONAS FLUORESCENS	1.9	X-RAY DIFFRACTION	98.2
2VSS	LYASE	04/29/08	Wild-type Hydroxycinnamoyl-CoA hydratase lyase in complex with acetyl- CoA and vanillin	PSEUDOMONAS FLUORESCENS	2.22	X-RAY DIFFRACTION	98.6
6L3P	LYASE	10/14/19	Crystal strcuture of Feruloyl-CoA hydratase lyase(FCHL) complexed with CoA	Pseudomonas putida KT2440	2.5	X-RAY DIFFRACTION	93.1

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 495 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG016253 (84 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PFLU3300 Search term: p-hydroxycinnamoyl CoA hydratase/lyase

Human Homologs

References