Pseudomonas putida HB3267, B479_09485 (prpB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown

Gene Feature Overview

Strain Pseudomonas putida HB3267
GCF_000325725.1|latest
Locus Tag
B479_09485
Name
prpB
Replicon chromosome
Genomic location 2107457 - 2108347 (+ strand)

Cross-References

RefSeq YP_007228744.1
GI 431801841
EC Number 4.1.3.30
Entrez 14288292
INSDC AGA72801.1
NCBI Locus Tag B479_09485
UniParc UPI00027BB869
UniProtKB Acc L0FJ87
UniProtKB ID L0FJ87_PSEPU
UniRef100 UniRef100_A0A099MWU7
UniRef50 UniRef50_Q56062
UniRef90 UniRef90_Q88KF6

Product

Feature Type CDS
Coding Frame 1
Product Name
2-methylisocitrate lyase
Synonyms
Evidence for Translation
Charge (pH 7) -4.89
Kyte-Doolittle Hydrophobicity Value 0.046
Molecular Weight (kDa) 31.8
Isoelectric Point (pI) 5.41

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6T5M LYASE 10/16/19 Crystal structure of 2-methylisocitrate lyase (PrpB) from Pseudomonas aeruginosa in complex with Mg(II)-pyruvate. Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.76 X-RAY DIFFRACTION 91.0
6T4V LYASE 10/15/19 Crystal structure of 2-methylisocitrate lyase (PrpB) from Pseudomonas aeruginosa in apo form. Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.807 X-RAY DIFFRACTION 91.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 335 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000769 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: B479_09485
Search term: prpB

Human Homologs

References

No references are associated with this feature.