Pseudomonas aeruginosa PA7, PSPA7_5554 (cntM)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Pseudomonas aeruginosa PA7
GCF_000017205.1|latest
Locus Tag
PSPA7_5554
Name
cntM
Synonym: zrmC
Replicon chromosome
Genomic location 5717872 - 5719173 (- strand)
Commentpart of the Zur-regulated metallophore operon known as cntOLMI (PMID:29214991) or zrmABCD(PMID:28898501).

Cross-References

RefSeq YP_001350875.1
GI 152984564
Entrez 5358302
INSDC ABR81747.1
NCBI Locus Tag PSPA7_5554
UniParc UPI0000D7272F
UniProtKB Acc A6VCU0
UniProtKB ID A6VCU0_PSEA7
UniRef100 UniRef100_A6VCU0
UniRef50 UniRef50_Q9HUX5
UniRef90 UniRef90_Q9HUX5

Product

Feature Type CDS
Coding Frame 1
Product Name
CntM
Product Name Confidence: Class 3
Synonyms hypothetical protein
Evidence for Translation
Charge (pH 7) 5.41
Kyte-Doolittle Hydrophobicity Value -0.054
Molecular Weight (kDa) 48066.7
Isoelectric Point (pI) 8.30

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6PBT BIOSYNTHETIC PROTEIN 06/14/19 Pseudopaline Dehydrogenase with (R)-Pseudopaline Soaked 2 hours Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.18 X-RAY DIFFRACTION 90.5
6PBM oxidoreductase, biosynthetic protein 06/14/19 Pseudopaline Dehydrogenase with NADP+ bound Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.57 X-RAY DIFFRACTION 90.5
6PBN BIOSYNTHETIC PROTEIN 06/14/19 Pseudopaline Dehydrogenase with (R)-Pseudopaline Soaked 1 hour Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.65 X-RAY DIFFRACTION 90.5
6PBP BIOSYNTHETIC PROTEIN 06/14/19 Pseudopaline Dehydrogenase with (S)-Pseudopaline Soaked 1 hour Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.64 X-RAY DIFFRACTION 90.5
6C4N OXIDOREDUCTASE 01/12/18 Pseudopaline dehydrogenase (PaODH) - NADP+ bound Pseudomonas aeruginosa PAO1 1.95 X-RAY DIFFRACTION 90.5

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 11 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004466 (299 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PSPA7_5554
Search term: cntM
Search term: CntM

Human Homologs

References

Growth of Pseudomonas aeruginosa in zinc poor environments is promoted by a nicotianamine-related metallophore.
Mastropasqua MC, D'Orazio M, Cerasi M, Pacello F, Gismondi A, Canini A, Canuti L, Consalvo A, Ciavardelli D, Chirullo B, Pasquali P, Battistoni A
Mol Microbiol 2017 Nov;106(4):543-561
PubMed ID: 28898501
Pseudomonas aeruginosa zinc uptake in chelating environment is primarily mediated by the metallophore pseudopaline.
Lhospice S, Gomez NO, Ouerdane L, Brutesco C, Ghssein G, Hajjar C, Liratni A, Wang S, Richaud P, Bleves S, Ball G, Borezée-Durant E, Lobinski R, Pignol D, Arnoux P, Voulhoux R
Sci Rep 2017 Dec 7;7(1):17132
PubMed ID: 29214991