Pseudomonas aeruginosa PA7, PSPA7_5647

Gene Feature Overview

Strain	Pseudomonas aeruginosa PA7 GCF_000017205.1|latest
Locus Tag	PSPA7_5647
Name
Replicon	chromosome
Genomic location	5809876 - 5810799 (+ strand)

Cross-References

RefSeq	YP_001350968.1
GI	152988985
Entrez	5358219
INSDC	ABR86168.1
NCBI Locus Tag	PSPA7_5647
UniParc	UPI0000D7277C
UniProtKB Acc	A6VD33
UniProtKB ID	A6VD33_PSEA7
UniRef100	UniRef100_A6VD33
UniRef50	UniRef50_Q9HUP2
UniRef90	UniRef90_Q9HUP2

Product

Feature Type	CDS
Coding Frame	1
Product Name	hypothetical protein
Synonyms
Evidence for Translation
Charge (pH 7)	3.58
Kyte-Doolittle Hydrophobicity Value	-0.261
Molecular Weight (kDa)	33.6
Isoelectric Point (pI)	8.25

Subcellular localization

Individual Mappings	Localization Confidence PMID Extracellular Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6UQW	HYDROLASE	10/21/19	Crystal structure of ChoE in complex with acetate and thiocholine	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.65	X-RAY DIFFRACTION	96.2
8D8W	HYDROLASE	06/09/22	Crystal structure of ChoE with Ser38 adopting alternative conformations	Pseudomonas aeruginosa	1.4	X-RAY DIFFRACTION	96.2
6UR1	HYDROLASE	10/21/19	Crystal structure of ChoE S38A mutant in complex with acetate and acetylthiocholine	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.42	X-RAY DIFFRACTION	95.8
6UQY	HYDROLASE	10/21/19	Crystal structure of ChoE H288N mutant in complex with acetylthiocholine	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.57	X-RAY DIFFRACTION	95.8
8D90	HYDROLASE	06/09/22	Crystal structure of ChoE N147A mutant in complex with bromide ions	Pseudomonas aeruginosa	1.82	X-RAY DIFFRACTION	95.8
6UR0	HYDROLASE	10/21/19	Crystal structure of ChoE D285N mutant acyl-enzyme	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.8	X-RAY DIFFRACTION	95.5
8D91	HYDROLASE	06/09/22	Crystal structure of ChoE in complex with acetate and tetraethylammonium (TEA)	Pseudomonas aeruginosa	1.49	X-RAY DIFFRACTION	96.2
8D8X	HYDROLASE	06/09/22	Crystal structure of ChoE in complex with acetate and thiocholine (crystal form 2)	Pseudomonas aeruginosa	1.36	X-RAY DIFFRACTION	96.2
8D8Y	HYDROLASE	06/09/22	Crystal structure of ChoE N147A mutant in complex with acetylthiocholine	Pseudomonas aeruginosa	1.54	X-RAY DIFFRACTION	95.8
6UQV	HYDROLASE	10/21/19	Crystal structure of ChoE, a bacterial acetylcholinesterase from Pseudomonas aeruginosa	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.35	X-RAY DIFFRACTION	96.2
8D8Z	HYDROLASE	06/09/22	Crystal structure of ChoE N147A mutant in complex with thiocholine and chloride	Pseudomonas aeruginosa	1.38	X-RAY DIFFRACTION	95.8
6UQX	HYDROLASE	10/21/19	Crystal structure of ChoE in complex with propionylthiocholine	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.85	X-RAY DIFFRACTION	96.2
6UQZ	HYDROLASE	10/21/19	Crystal structure of ChoE D285N mutant in complex with acetate and thiocholine	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.43	X-RAY DIFFRACTION	95.8

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 71 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004538 (814 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PSPA7_5647

Human Homologs

References