Pseudomonas stutzeri RCH2, Psest_3612

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown

Gene Feature Overview

Strain Pseudomonas stutzeri RCH2
GCF_000327065.1|latest
Locus Tag
Psest_3612
Name
Replicon chromosome
Genomic location 3774406 - 3778605 (- strand)

Cross-References

RefSeq YP_007241730.1
GI 431928696
EC Number 2.7.7.6
Entrez 14301459
NCBI Locus Tag Psest_3612

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA-directed RNA polymerase subunit beta'
Synonyms
Evidence for Translation
Charge (pH 7) -2.94
Kyte-Doolittle Hydrophobicity Value -0.222
Molecular Weight (kDa) 155013.0
Isoelectric Point (pI) 6.75

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7XL4 TRANSCRIPTION 04/21/22 Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (closed lobe) Pseudomonas aeruginosa PAO1 3.86 ELECTRON MICROSCOPY 92.8
7F0R TRANSCRIPTION/DNA 06/06/21 Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 5.8 ELECTRON MICROSCOPY 92.8
7XYA TRANSCRIPTION 06/01/22 The cryo-EM structure of an AlpA-loading complex Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 3.3 ELECTRON MICROSCOPY 92.9
7XYB TRANSCRIPTION 06/01/22 The cryo-EM structure of an AlpA-loaded complex Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 3.7 ELECTRON MICROSCOPY 92.9
7VF9 TRANSCRIPTION 09/10/21 Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes Pseudomonas aeruginosa PAO1 4.04 ELECTRON MICROSCOPY 92.8
7XL3 TRANSCRIPTION 04/21/22 Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (open lobe) Pseudomonas aeruginosa PAO1 3.13 ELECTRON MICROSCOPY 92.8

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 667 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001442 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: Psest_3612

Human Homologs

Ensembl 110, assembly GRCh38.p14
RNA polymerase III subunit A [Source:HGNC Symbol;Acc:HGNC:30074]
E-value: 3.0e-46
Percent Identity: 23.4

References

No references are associated with this feature.