Pseudomonas syringae pv. tomato DC3000, PSPTO_3977 (ruvB)

Gene Feature Overview

Strain	Pseudomonas syringae pv. tomato DC3000 (Buell et al., 2003) GCF_000007805.1|latest
Locus Tag	PSPTO_3977
NCBI Old Locus Tag	PSPTO3977
Name	ruvB Synonym: PSPTO3977
Replicon	chromosome
Genomic location	4483595 - 4484533 (- strand)

Cross-References

RefSeq	NP_793741.1
GI	28871122
Entrez	1185653
INSDC	AAO57436.1
NCBI Locus Tag	PSPTO_3977
NCBI Old Locus Tag	PSPTO3977
UniParc	UPI000023FEE8
UniProtKB Acc	Q87Y35
UniProtKB ID	RUVB_PSESM
UniRef100	UniRef100_Q87Y35
UniRef50	UniRef50_Q5SL87
UniRef90	UniRef90_Q48FC5

Product

Feature Type	CDS
Coding Frame	1
Product Name	Holliday junction DNA helicase RuvB
Synonyms
Evidence for Translation
Charge (pH 7)	-7.30
Kyte-Doolittle Hydrophobicity Value	-0.022
Molecular Weight (kDa)	34.3
Isoelectric Point (pI)	5.24

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6BLB	HYDROLASE	11/09/17	1.88 Angstrom Resolution Crystal Structure Holliday Junction ATP-dependent DNA Helicase (RuvB) from Pseudomonas aeruginosa in Complex with ADP	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	1.88	X-RAY DIFFRACTION	90.9
7X7Q	RECOMBINATION/DNA	03/10/22	CryoEM structure of RuvA-RuvB-Holliday junction complex	Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	7.02	ELECTRON MICROSCOPY	90.9
7X7P	MOTOR PROTEIN/DNA	03/10/22	CryoEM structure of dsDNA-RuvB-RuvA domain3 complex	Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT	7.02	ELECTRON MICROSCOPY	91.5
7X5B	MOTOR PROTEIN	03/04/22	Crystal structure of RuvB	Pseudomonas aeruginosa PAO1	2.16	X-RAY DIFFRACTION	90.6

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 585 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000932 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PSPTO_3977 Search term: ruvB Search term: Holliday junction DNA helicase RuvB

Human Homologs

References