Pseudomonas aeruginosa LESB58, PALES_12581 (lasB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa LESB58
GCF_000026645.1|latest
Locus Tag
PALES_12581
Name
lasB
Replicon chromosome
Genomic location 1367021 - 1368517 (+ strand)

Cross-References

RefSeq YP_002438864
GI 218890000
Entrez 7180866

Product

Feature Type CDS
Coding Frame 1
Product Name
elastase LasB
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -1.48
Kyte-Doolittle Hydrophobicity Value -0.271
Molecular Weight (kDa) 53.7
Isoelectric Point (pI) 6.74

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7QH1 HYDROLASE 12/10/21 Discovery and development of a novel inhaled antivirulence therapy for the treatment of Pseudomonas aeruginosa infections in patients with chronic respiratory disease Pseudomonas aeruginosa 2.74 X-RAY DIFFRACTION 100.0
3DBK HYDROLASE 06/01/08 Pseudomonas aeruginosa elastase with phosphoramidon Pseudomonas aeruginosa 1.4 X-RAY DIFFRACTION 100.0
8CR3 HYDROLASE 03/07/23 Crystal structure of recombinant LasB from Pseudomonas aeruginosa PAO1 Pseudomonas aeruginosa PAO1 1.12 X-RAY DIFFRACTION 98.4
8CR7 HYDROLASE 03/07/23 Crystal structure of recombinant LasB from Pseudomonas aeruginosa PA7 Pseudomonas aeruginosa PA7 1.5 X-RAY DIFFRACTION 95.9
1EZM HYDROLASE 01/13/92 THREE-DIMENSIONAL STRUCTURE OF THE ELASTASE OF PSEUDOMONAS AERUGINOSA AT 1.5 ANGSTROMS RESOLUTION Pseudomonas aeruginosa 1.5 X-RAY DIFFRACTION 100.0
6FZX HYDROLASE 03/15/18 LasB, hydroxymate Inhibitor Complex Pseudomonas aeruginosa 2.1 X-RAY DIFFRACTION 100.0
1U4G HYDROLASE 07/25/04 Elastase of Pseudomonas aeruginosa with an inhibitor Pseudomonas aeruginosa 1.4 X-RAY DIFFRACTION 100.0
6F8B HYDROLASE 12/12/17 LasB bound to thiol based inhibitor Pseudomonas aeruginosa 1.3 X-RAY DIFFRACTION 100.0
8CR4 HYDROLASE 03/07/23 Crystal structure of recombinant LasBArtif from Pseudomonas aeruginosa AZPAE14816 Pseudomonas aeruginosa 0.91 X-RAY DIFFRACTION 94.9
7AJR HYDROLASE 09/29/20 Virtual screening approach leading to the identification of a novel and tractable series of Pseudomonas aeruginosa elastase inhibitors Pseudomonas aeruginosa 1.75 X-RAY DIFFRACTION 100.0
4K89 HYDROLASE 04/18/13 Crystal structure of Pseudomonas aeruginosa strain K solvent tolerant elastase Pseudomonas aeruginosa 1.39 X-RAY DIFFRACTION 99.7
7NLM HYDROLASE 02/22/21 LasB, N-aryl-2-butylmercaptoacetamide Pseudomonas aeruginosa (strain UCBPP-PA14) 1.65 X-RAY DIFFRACTION 98.7
7OC7 HYDROLASE 04/26/21 LasB, alpha-alkyl-N-aryl mercaptoacetamide Pseudomonas aeruginosa 1.95 X-RAY DIFFRACTION 98.7
7NLK HYDROLASE 02/22/21 LasB, N-aryl-2-butylmercaptoacetamide Pseudomonas aeruginosa (strain UCBPP-PA14) 1.7 X-RAY DIFFRACTION 98.7

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
N-(1-Carboxy-3-Phenylpropyl)Phenylalanyl-Alpha-Asparagine DB02307 DrugBank 5.1.4 P14756 Elastase 100.0 498 6.5e-289

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 114 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001959 (310 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PALES_12581
Search term: lasB
Search term: elastase LasB

Human Homologs

References

No references are associated with this feature.