Pseudomonas aeruginosa PAO1, PA0317

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0317
Name
Synonym: D2HGDH
Replicon chromosome
Genomic location 356681 - 358075 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249008.1
GI 15595514
Affymetrix PA0317_at
Entrez 878323
GenBank AAG03706.1
INSDC AAG03706.1
NCBI Locus Tag PA0317
protein_id(GenBank) gb|AAG03706.1|AE004469_11|gnl|PseudoCAP|PA0317
TIGR NTL03PA00318
UniParc UPI00000C5000
UniProtKB Acc Q9I6H4
UniProtKB ID Q9I6H4_PSEAE
UniRef100 UniRef100_Q9I6H4
UniRef50 UniRef50_F5SNV8
UniRef90 UniRef90_Q9I6H4

Product

Feature Type CDS
Coding Frame 1
Product Name
D-2-hydroxyglutarate dehydrogenase
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -13.18
Kyte-Doolittle Hydrophobicity Value -0.079
Molecular Weight (kDa) 51.3
Isoelectric Point (pI) 4.88

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 549 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000314 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0317

Human Homologs

Ensembl 86, assembly GRCh38.p7
D-2-hydroxyglutarate dehydrogenase [Source:HGNC Symbol;Acc:HGNC:28358]
E-value: 2.3e-70
Percent Identity: 34.4

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
PubMed ID: 28827360