Pseudomonas aeruginosa PAO1, PA0317

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0317
Name
Synonym: D2HGDH
Replicon chromosome
Genomic location 356681 - 358075 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249008.1
GI 15595514
Affymetrix PA0317_at
Entrez 878323
GenBank AAG03706.1
INSDC AAG03706.1
NCBI Locus Tag PA0317
protein_id(GenBank) gb|AAG03706.1|AE004469_11|gnl|PseudoCAP|PA0317
TIGR NTL03PA00318
UniParc UPI00000C5000
UniProtKB Acc Q9I6H4
UniProtKB ID Q9I6H4_PSEAE
UniRef100 UniRef100_Q9I6H4
UniRef50 UniRef50_F5SNV8
UniRef90 UniRef90_Q9I6H4

Product

Feature Type CDS
Coding Frame 1
Product Name
D-2-hydroxyglutarate dehydrogenase
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -13.18
Kyte-Doolittle Hydrophobicity Value -0.079
Molecular Weight (kDa) 51.3
Isoelectric Point (pI) 4.88

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 549 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000314 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0317

Human Homologs

Ensembl 86, assembly GRCh38.p7
D-2-hydroxyglutarate dehydrogenase [Source:HGNC Symbol;Acc:HGNC:28358]
E-value: 2.3e-70
Percent Identity: 34.4

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Coupling between d-3-phosphoglycerate dehydrogenase and d-2-hydroxyglutarate dehydrogenase drives bacterial l-serine synthesis.
Zhang W, Zhang M, Gao C, Zhang Y, Ge Y, Guo S, Guo X, Zhou Z, Liu Q, Zhang Y, Ma C, Tao F, Xu P.
Proc Natl Acad Sci U S A. 2017 Sep 5;114(36):E7574-E7582.
PubMed ID: 28827360